Guo Keyu, Liu Tong, Li Shun, Zhu Daobin, Fan Yuanyuan, Fan Rangrang, Ding Changchun, Jin Wei, Hu Junshan
School of Science, Key Laboratory of High-Performance Scientific Computation, Xihua University, Chengdu, 610039, China.
Department of Neurosurgery and Institute of Neurosurgery, West China Hospital, Sichuan University, Chengdu, 610041, China.
Mikrochim Acta. 2025 Jul 10;192(8):493. doi: 10.1007/s00604-025-07309-4.
An upconversion luminescence (UCL) nanoprobe is reported for the rapid detection of hypochlorite ions (ClO) in complex environments. The probe is fabricated by sensitizing rhodamine B (RhB) on the surface of core@shell upconversion nanoparticles (C/S UCNPs) with the bridging of polyethylene glycol (PEG) molecules. Under 980 nm excitation, the UCL of C/S UCNPs is quenched by RhB through fluorescence resonance energy transfer (FRET), which can be restored by the oxidative cleavage of RhB induced by ClO. It is observed that the green emission intensity of RhB-modified C/S UCNPs increases linearly with the concentration of ClO, enabling quantitative detection of ClO. Moreover, the excitation of C/S UCNPs requires only a low power density (0.09 W/cm), which minimizes the photodegradation of RhB and stabilizes the FRET process. Under optimized conditions, the detection limit for ClO ions in tap water was 5 µg mL⁻.
报道了一种用于在复杂环境中快速检测次氯酸根离子（ClO）的上转换发光（UCL）纳米探针。该探针通过聚乙二醇（PEG）分子桥接在核壳型上转换纳米颗粒（C/S UCNPs）表面敏化罗丹明B（RhB）制备而成。在980 nm激发下，C/S UCNPs的上转换发光通过荧光共振能量转移（FRET）被RhB猝灭，而ClO诱导的RhB氧化裂解可使其恢复。观察到RhB修饰的C/S UCNPs的绿色发射强度随ClO浓度呈线性增加，从而能够对ClO进行定量检测。此外，C/S UCNPs的激发仅需低功率密度（0.09 W/cm），这使RhB的光降解最小化并稳定了FRET过程。在优化条件下，自来水中ClO离子的检测限为5 μg mL⁻。
