Gao Li, Li Le-Le, Wang Xiaolong, Wu Peiwen, Cao Yang, Liang Bo, Li Xin, Lin Yuanwei, Lu Yi, Guo Xuefeng
Center for Nanochemistry, Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R. China.
Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
Chem Sci. 2015 Apr 1;6(4):2469-2473. doi: 10.1039/C4SC03612C. Epub 2015 Feb 5.
Many metal ions are present in biology and in the human body in trace amounts. Despite numerous efforts, metal sensors with ultrahigh sensitivity (< a few picomolar) are rarely achieved. Here, we describe a platform method that integrates a Cu-dependent DNAzyme into graphene-molecule junctions and its application for direct detection of paramagnetic Cu with femtomolar sensitivity and high selectivity. Since DNAzymes specific for other metal ions can be obtained through in vitro selection, the method demonstrated here can be applied to the detection of a broad range of other metal ions.
许多金属离子以痕量存在于生物学和人体中。尽管付出了诸多努力，但很少能实现具有超高灵敏度（< 几皮摩尔）的金属传感器。在此，我们描述了一种将铜依赖性脱氧核酶整合到石墨烯 - 分子结中的平台方法及其用于飞摩尔灵敏度和高选择性直接检测顺磁性铜的应用。由于可通过体外筛选获得对其他金属离子具有特异性的脱氧核酶，因此本文展示的方法可应用于检测多种其他金属离子。
