Jiao Shufei, Zhang Ruirui, Yin Yanzhen, Zhong Shuming, Liu Zijie, Zheng Yunying, Hu Xiaoxi, Liang Xingtang, Huang Zuqiang
Qinzhou Key Laboratory of Biowaste Resources for Selenium-enriched Functional Utilization, College of Petroleum and Chemical Engineering, Beibu Gulf University Qinzhou 535011 China
School of Chemistry and Chemical Engineering, Guangxi University Nanning 530004 China
RSC Adv. 2019 Sep 13;9(49):28814-28822. doi: 10.1039/c9ra05775g. eCollection 2019 Sep 9.
Excessive reactive oxygen free radicals (ROS) are the main cause of various oxidative diseases. It is of great significance to develop antioxidant drugs that can intelligently regulate free radical concentrations. The biomimetic simulation of glutathione peroxidase (GPx) can provide an important theoretical basis for the development of antioxidant drugs. In order to explore a simple and efficient strategy for constructing biomimetic GPx, a microgel biomimetic GPx (PNTegel) with temperature responsive catalytic activity was prepared by a one-pot synthesis method. The PNTegel, with typical enzymatic catalytic characteristics, exhibited a maximum catalytic activity at 37 °C ( = 11.51 mM min). The investigation of the catalytic mechanism of PNTegel suggested that the binding of different hydrophobic substrates to PNTegel was altered by the change of hydrophobicity of poly(-isopropylacrylamide) (PNIPAM) in the microgel scaffold of PNTegel during the temperature response process. The change of hydrophobicity was the main factor for regulating the catalytic activity of PNTegel, which resulted in a temperature responsive catalytic behavior of PNTegel. This new strategy for the simple and efficient construction of biomimetic GPx by a one-pot method provides important theoretical support for exploring the preparation of highly effective antioxidant drugs.
过量的活性氧自由基（ROS）是各种氧化疾病的主要原因。开发能够智能调节自由基浓度的抗氧化药物具有重要意义。谷胱甘肽过氧化物酶（GPx）的仿生模拟可为抗氧化药物的开发提供重要的理论依据。为了探索一种简单高效的构建仿生GPx的策略，采用一锅合成法制备了具有温度响应催化活性的微凝胶仿生GPx（PNTegel）。PNTegel具有典型的酶催化特性，在37℃时表现出最大催化活性（ = 11.51 mM min）。对PNTegel催化机制的研究表明，在温度响应过程中，PNTegel微凝胶支架中聚（N-异丙基丙烯酰胺）（PNIPAM）疏水性的变化改变了不同疏水底物与PNTegel的结合。疏水性的变化是调节PNTegel催化活性的主要因素，这导致了PNTegel的温度响应催化行为。这种通过一锅法简单高效构建仿生GPx的新策略为探索高效抗氧化药物的制备提供了重要的理论支持。
