Pandey Vivek, Tripathi Vinay Kumar, Singh Keshav Kumar, Bhatia Tejasvi, Upadhyay Nitesh Kumar, Goyal Bela, Pandey Gajanan, Hwang Inho, Tandon Poonam
Department of Physics, University of Lucknow Lucknow-226007 India.
Department of Animal Science and Biotechnology, Chonbuk National University Jeonju 561-756 Republic of Korea.
RSC Adv. 2019 Sep 11;9(49):28510-28524. doi: 10.1039/c9ra05651c. eCollection 2019 Sep 9.
Nanoparticles having strong optical and electronic properties are the most widely used materials in sensor development. Since the target analyte interacts directly with the surface of the material, the choice of ligand for functionalizing the surface of the material is the key for its further applications. The functionalized surface of the material makes it suitable for required applications as it controls the size of the particle during its growth from the solution phase. Biomolecule capped nanomaterials are favourable for various applications in bio-sensing. In the present work, an attempt has been made to explore the biologically active molecule imidazole as capping agent for ZnS semiconductor nanoparticles or quantum dots (QDs). This work explores the possibility of replacing conventional thiol-zinc bonding and hence paves new pathways for biomolecules having the possibility of being efficient capping agents. Computational chemistry has been used to study the mechanism of bonding between one of the nitrogen atoms of imidazole and the zinc ion of the ZnS QDs. The quantum chemical insight not only explores the most spontaneous interaction of zinc ion and imidazole molecule so as to act as an efficient capping agent but also explains the probable bonding site for nitrogen-zinc chemistry. The tailormade Mn doped ZnS QDs are one of the most promising materials for probe and sensor development. The ZnS core having non-toxicity and the emission in longer wavelength due to manganese makes this material highly useful biologically. The aqueous route of synthesis has been employed to obtain a highly homogeneous and pure material which was further characterized by UV (Ultra Violet spectroscopy), Spectrofluorometer, Transmission Electron Microscope and X-ray Diffraction. The toxicity at the cellular and genetic levels was also investigated to prove the potential of the imidazole capped Mn doped ZnS QD as a biocompatible material.
具有强光学和电子性质的纳米颗粒是传感器开发中应用最广泛的材料。由于目标分析物直接与材料表面相互作用，选择用于功能化材料表面的配体是其进一步应用的关键。材料的功能化表面使其适用于所需应用，因为它在从溶液相生长过程中控制颗粒大小。生物分子封端的纳米材料有利于生物传感中的各种应用。在本工作中，已尝试探索将生物活性分子咪唑用作硫化锌半导体纳米颗粒或量子点（QD）的封端剂。这项工作探索了取代传统硫醇 - 锌键合的可能性，从而为有可能成为高效封端剂的生物分子开辟了新途径。计算化学已被用于研究咪唑的一个氮原子与硫化锌量子点的锌离子之间的键合机制。量子化学见解不仅探索了锌离子与咪唑分子最自发的相互作用，以便作为一种有效的封端剂，而且还解释了氮 - 锌化学的可能键合位点。特制的锰掺杂硫化锌量子点是用于探针和传感器开发最有前途的材料之一。具有无毒特性的硫化锌核心以及由于锰导致的较长波长发射使得这种材料在生物学上非常有用。已采用水相合成路线获得高度均匀和纯净的材料，该材料进一步通过紫外（紫外光谱）、荧光分光光度计、透射电子显微镜和X射线衍射进行表征。还研究了细胞和遗传水平的毒性，以证明咪唑封端的锰掺杂硫化锌量子点作为生物相容性材料的潜力。
