Han Baoqing, Yu Biao, Liu Lu, Xiu Yi, Wang Hongsu
College of Food Sciences and Engineering, Jilin University Changchun 130062 Jilin Province China
Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University Changchun 130062 Jilin Province China.
RSC Adv. 2019 Aug 15;9(44):25739-25749. doi: 10.1039/c9ra03595h. eCollection 2019 Aug 13.
Perilla oil (PO) and linseed oil (LO) are very rich in nutrients and have great potential market value. Using the Cremophor RH40-Span80 preparation system to make a perilla oil nanoemulsion (PON) or linseed oil nanoemulsion (LON) can improve the bioavailability and stability of these oils. The effect of different reaction conditions on the stability of the emulsion was investigated. The results showed that the PON and LON have good stability at pH ≥ 7, different storage temperatures (4 °C, 25 °C, 37 °C and 55 °C) and different NaCl concentrations (0, 2, 4, 6, 8 M). Meanwhile, it was found that the content of the lipid peroxidation product malondialdehyde (MDA) in the nanoemulsion did not change significantly over 7 days, further demonstrating the stability of the nanoemulsion. Through anti-inflammatory and antibacterial tests, it was found that the PON and LON have an effective inhibitory effect on inflammation; moreover, the PON inhibits the growth of , and , and the LON has an inhibitory effect on and (inhibition zone > 10 mm). In addition, we found that there were no pathological differences in the heart, liver, spleen and kidney of Kunming mice between the PO and PON groups and the LO and LON groups. Furthermore, after intraperitoneal injection of P 407 into mice, the comparison between PON and PO and between LON and LO showed that the blood lipid levels of the mice in the PON and LON treatment groups increased, indicating that the absorption capacity of the small intestine of the mice for the PON and LON was enhanced. Therefore, the preparation of the PON and LON has good development prospects and opens up opportunities in the development of the food industry.
紫苏油（PO）和亚麻籽油（LO）营养丰富，具有很大的潜在市场价值。使用聚氧乙烯蓖麻油RH40-司盘80制备体系制备紫苏油纳米乳（PON）或亚麻籽油纳米乳（LON），可提高这些油的生物利用度和稳定性。研究了不同反应条件对乳液稳定性的影响。结果表明，PON和LON在pH≥7、不同储存温度（4℃、25℃、37℃和55℃）以及不同NaCl浓度（0、2、4、6、8M）下具有良好的稳定性。同时，发现纳米乳中脂质过氧化产物丙二醛（MDA）的含量在7天内无明显变化，进一步证明了纳米乳的稳定性。通过抗炎和抗菌试验发现，PON和LON对炎症有有效抑制作用；此外，PON对金黄色葡萄球菌、大肠杆菌和白色念珠菌的生长有抑制作用，LON对大肠杆菌和白色念珠菌有抑制作用（抑菌圈>10mm）。此外，我们发现PO和PON组以及LO和LON组的昆明小鼠心脏、肝脏、脾脏和肾脏无病理差异。此外，给小鼠腹腔注射P407后，PON与PO、LON与LO之间的比较表明，PON和LON处理组小鼠的血脂水平升高，表明小鼠小肠对PON和LON的吸收能力增强。因此，PON和LON的制备具有良好的发展前景，为食品工业的发展开辟了机遇。
