Jiao Mengya, Chen Lei, He Yinru, Wu Lirong, Mei Hanfang
Department of Biochemistry and Molecular Biology, School of Life Science and Biopharmaceuticals, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center Guangzhou Guangdong 510006 China.
Department of Dental Emergency, Stomatological Hospital of Southern Medical University, Southern Medical University Guangzhou Guangdong 510280 China.
RSC Adv. 2019 Sep 27;9(52):30545-30555. doi: 10.1039/c9ra05854k. eCollection 2019 Sep 23.
Housefly larvae (HL) powder was used to cure wounds centuries ago for its good nutritional and pharmacological values. At present, most of the medical studies are about the crude extracts of HL, while the specific pharmacological material basis is still unclear. We ground third-instar larvae into a powder, degreasing and preparing the protein extract. The protein extract was subjected to enzymatic hydrolysis, and the enzymatic hydrolysis products were identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). We identified a variety of highly trusted proteins (false discovery rate is less than or equal to 1%), including catalysis-related proteins, antioxidant proteins and antimicrobial peptides, which may be closely related to the anti-tumor, anti-bacterial, anti-oxidant and other pharmacological effects of HL. We identified the amino acid sequences of these proteins, and further confirmed HL's protective effect on APP/PS1 transgenic Alzheimer's mice. The results of this work provide material basis for further medical research on HL.
几个世纪前，家蝇幼虫（HL）粉因其良好的营养和药理价值被用于治疗伤口。目前，大多数医学研究是关于HL的粗提物，而具体的药理物质基础仍不清楚。我们将三龄幼虫磨成粉末，脱脂并制备蛋白质提取物。对蛋白质提取物进行酶解，酶解产物通过液相色谱-串联质谱（LC-MS/MS）进行鉴定。我们鉴定出多种高度可信的蛋白质（错误发现率小于或等于1%），包括催化相关蛋白、抗氧化蛋白和抗菌肽，它们可能与HL的抗肿瘤、抗菌、抗氧化等药理作用密切相关。我们鉴定了这些蛋白质的氨基酸序列，并进一步证实了HL对APP/PS1转基因阿尔茨海默病小鼠的保护作用。这项工作的结果为HL的进一步医学研究提供了物质基础。
