电针对 APP/PS1 小鼠学习记忆能力的改善作用与其对 N- 乙酰天门冬氨酸和谷氨酸代谢的影响
Electroacupuncture ameliorate learning and memory by improving N-acetylaspartate and glutamate metabolism in APP/PS1 mice.
机构信息
Fujian Collaborative Innovation Center for Rehabilitation Technology, Fuzhou, 350122, People's Republic of China.
TCM Rehabilitation Research Center of SATCM, Fuzhou, 350122, People's Republic of China.
出版信息
Biol Res. 2018 Jul 6;51(1):21. doi: 10.1186/s40659-018-0166-7.
OBJECTIVE
To explore the precise mechanism of electroacupuncture (EA) to delay cognitive impairment in Alzheimer disease.
METHODS
N-Acetylaspartate (NAA), glutamate (Glu) and myoinositol (mI) metabolism were measured by magnetic resonance spectroscopy, learning and memory of APP/PS1 mouse was evaluated by the Morris water maze test and the step-down avoidance test, neuron survival number and neuronal structure in the hippocampus were observed by Nissl staining, and BDNF and phosphorylated TrkB detected by Western blot.
RESULTS
EA at DU20 acupuncture significantly improve learning and memory in behavioral tests, up-regulate NAA, Glu and mI metabolism, increase the surviving neurons in hippocampus, and promote the expression of BDNF and TrkB in the APP/PS1 transgenic mice.
CONCLUSION
These findings suggested that EA is a potential therapeutic for ameliorate cognitive dysfunction, and it might be due to EA could improve NAA and Glu metabolism by upregulation of BDNF in APP/PS1 mice.
目的
探索电针对阿尔茨海默病认知障碍的精确作用机制。
方法
通过磁共振波谱测量 N-乙酰天门冬氨酸（NAA）、谷氨酸（Glu）和肌醇（mI）代谢，通过 Morris 水迷宫试验和避台试验评估 APP/PS1 小鼠的学习和记忆能力，通过尼氏染色观察海马区神经元存活数量和神经元结构，通过 Western blot 检测 BDNF 和磷酸化 TrkB。
结果
电针 DU20 穴位可明显改善行为学测试中的学习和记忆能力，上调 NAA、Glu 和 mI 代谢，增加海马区存活神经元，并促进 APP/PS1 转基因小鼠中 BDNF 和 TrkB 的表达。
结论
这些发现表明电针可能是改善认知功能障碍的一种潜在治疗方法，这可能是由于电针通过上调 APP/PS1 小鼠中的 BDNF 来改善 NAA 和 Glu 代谢。
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