Despite the wide range of available antibiotics, food borne bacteria demonstrate a huge spectrum of resistance. The current study aims to use natural components such as essential oils (EOs), chitosan, and nano-chitosan that have very influential antibacterial properties with novel technologies like chitosan solution/film loaded with EOs against multi-drug resistant bacteria. Two strains of O157:H7 and three strains of were used to estimate antibiotics resistance. Ten EOs and their mixture, chitosan, nano-chitosan, chitosan plus EO solutions, and biodegradable chitosan film enriched with EOs were tested as antibacterial agents against pathogenic bacterial strains. Results showed that O157:H7 51,659 and 19,116 relatively exhibited considerable resistance to more than one single antibiotic. Turmeric, cumin, pepper black, and marjoram did not show any inhibition zone against ; Whereas, clove, thyme, cinnamon, and garlic EOs exhibited high antibacterial activity against with minimum inhibitory concentration (MIC) of 250-400 μl 100 ml and against O157:H7 with an MIC of 350-500 μl 100 ml, respectively. Among combinations, clove, and thyme EOs showed the highest antibacterial activity against O157:H7 with MIC of 170 μl 100 ml, and the combination of cinnamon and clove EOs showed the strongest antibacterial activity against with an MIC of 120 μl 100 ml. Both chitosan and nano-chitosan showed a promising potential as an antibacterial agent against pathogenic bacteria as their MICs were relatively lower against than for O157:H7. Chitosan combined with each of cinnamon, clove, and thyme oil have a more effective antibacterial activity against and O157:H7 than the mixture of oils alone. Furthermore, the use of either chitosan solution or biodegradable chitosan film loaded with a combination of clove and thyme EOs had the strongest antibacterial activity against and O157:H7. However, chitosan film without EOs did not exhibit an inhibition zone against the tested bacterial strains.