Sehsah Mohamed D, El-Kot Gabr A, El-Nogoumy Baher A, Alorabi Mohammed, El-Shehawi Ahmed M, Salama Nagwa H, El-Tahan Amira M
Maize and Sugar Crops Diseases Research Department, Plant Pathology Research Institution, Agricultural Research Center, Giza, Egypt.
Agriculture Botany Department, Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt.
Saudi J Biol Sci. 2022 Apr;29(4):2219-2229. doi: 10.1016/j.sjbs.2021.11.039. Epub 2021 Nov 24.
Cercospora leaf spot caused by are among the most dangerous plant diseases on sugar beet plants. It causes heavy economic losses, whether on the yield of roots, the percentage of sugar in them, or the quality of sugar produced. In addition to the economic cost caused by chemical control, these chemical pesticides cause an imbalance in the ecosystem and harm the health of humans and animals. In an attempt to search for a safer method than pesticides and environmentally friendly, an evaluation of using biocontrol agents, as cell suspension (10 cell/ml), was conducted in this study. Seeds extract of with two concentrations (25 and 50 g/L) and potassium bicarbonate at (5 and10 g/L (compared to fungicide Montoro 30% EC (Propiconazole 15% + Difenoconazole 15%). The evaluation results for twenty-five sugar beet varieties showed a significant discrepancy between these varieties in the extent of their susceptibility to infection with the disease under investigation. , induced an antagonist to , and both seeds extract and potassium bicarbonate significantly reduced the linear growth of this pathogen. Under field conditions, the treatments used have given positive results in controlling Cercospora leaf spots. They significantly decreased the severity of disease and prevented from creating conidiophores and conidiospores, along with examining their cell walls with the formation of plasmolysis of the fungus cells and reducing both the number and diameter of the spots on the surface leaves; this was demonstrated using a scanning electron microscope (SEM). It is worth noting that the best results obtained were most often when treated with seeds extract, followed by potassium bicarbonate, then cell suspension of In addition, the percentage of the content of beet roots from total soluble solids and sucrose has improved significantly due to spraying sugar beet plants with the substances mentioned earlier. These treatments also contributed to a significant improvement in the enzymes polyphenol oxidase, peroxidase, and phenylalanine ammonia-lyase.
由[病原菌名称未给出]引起的尾孢叶斑病是甜菜植株上最危险的植物病害之一。它会造成严重的经济损失,无论是在根产量、根中的含糖百分比还是所产糖的质量方面。除了化学防治造成的经济成本外,这些化学农药还会导致生态系统失衡,并危害人类和动物的健康。为了寻找一种比农药更安全且环保的方法,本研究对使用作为细胞悬浮液(10个细胞/毫升)的生物防治剂进行了评估。[植物名称未给出]种子提取物有两种浓度(25克/升和50克/升)以及碳酸氢钾(5克/升和10克/升)(与30%乳油杀菌剂Montoro(丙环唑15% + 苯醚甲环唑15%)相比)。对25个甜菜品种的评估结果表明,这些品种在对所研究病害感染的易感性程度上存在显著差异。[诱导物名称未给出]诱导产生了对[病原菌名称未给出]的拮抗剂,并且[植物名称未给出]种子提取物和碳酸氢钾都显著降低了这种病原菌的线性生长。在田间条件下,所使用的处理方法在防治尾孢叶斑病方面取得了积极成果。它们显著降低了病害的严重程度,阻止[病原菌名称未给出]产生分生孢子梗和分生孢子,同时通过扫描电子显微镜(SEM)观察到其真菌细胞壁形成质壁分离,减少了叶片表面病斑的数量和直径。值得注意的是,最常获得的最佳结果是用[植物名称未给出]种子提取物处理,其次是碳酸氢钾,然后是[生物防治剂名称未给出]的细胞悬浮液。此外,由于用上述物质喷洒甜菜植株,甜菜根中总可溶性固形物和蔗糖的含量百分比有了显著提高。这些处理方法还显著改善了多酚氧化酶、过氧化物酶和苯丙氨酸解氨酶的活性。