Srinivasa Chandrashekar, Umesha Sharanaiah, Pradeep Sushma, Ramu Ramith, Gopinath S M, Ansari Mohammad Azam, Alomary Mohammad N, Ali Abuzer, Ahmad Wasim, Kollur Shiva Prasad, Shivamallu Chandan
Department of Studies in Biotechnology, Davangere University, Shivagangotri, Davangere 577002, Karnataka, India.
Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore 570 005, Karnataka, India.
Saudi J Biol Sci. 2022 Apr;29(4):2253-2261. doi: 10.1016/j.sjbs.2021.11.067. Epub 2021 Dec 3.
Bacterial spot disease of tomato was identified a few decades ago. The varying point of entry for the pathogen has enhanced the complexities for designing an effective disease management method. Recent advances in the use of inducers to elicit immune response in order to prepare the plant for a future attack by the pathogen are similar to the vaccination that is undertaken in humans and is a trending technology.
The present study aims at understanding the effect of salicylic acid (SA) for management of bacterial spot disease caused by
The study evaluated the effects of SA on the disease incidence along with seed germination and seedling vigor in two cultivars of tomato namely, Sun hybrid- resistant variety (R) and Quality- highly susceptible variety(HS).
The germination and seedling vigor, which was reduced in case of HS variety in comparison with that of the R variety, was notably improved after seed priming in both the cultivars. The antioxidant enzyme activities of ascorbate peroxidise (APx), catalase (CAT) and glutathione reductase (GR) were assessed which showed that the R variety reached maximum activity at 18 h post inoculation (hpi), 24 hpi and 21 hpi, respectively, whereas the HS variety reached maximum at 30 hpi for APx and 36 hpi for CAT and GR activities. The transcript accumulation using qRT-PCR was also evaluated showing mRNA accumulation was maximum in the R cultivar after SA priming at 1.5 mM concentration.
the present study demonstrates the potential benefits of seed priming with SA to effectively elicit defence response in tomato seedlings against the bacterial spot disease.
番茄细菌性斑点病在几十年前就已被确认。病原体不同的侵入点增加了设计有效病害管理方法的复杂性。近年来，利用诱导剂引发免疫反应以使植物为未来病原体的攻击做好准备的研究进展，类似于人类进行的疫苗接种，是一项热门技术。
本研究旨在了解水杨酸（SA）对由[病原体名称缺失]引起的番茄细菌性斑点病的防治效果。
该研究评估了SA对两个番茄品种（即抗性品种Sun杂交种（R）和高感品种Quality（HS））的发病率以及种子发芽和幼苗活力的影响。
与R品种相比，HS品种的发芽和幼苗活力降低，但在两个品种进行种子引发处理后均有显著改善。对抗坏血酸过氧化物酶（APx）、过氧化氢酶（CAT）和谷胱甘肽还原酶（GR）的抗氧化酶活性进行了评估，结果表明，R品种在接种后18小时（hpi）、24 hpi和21 hpi分别达到最大活性，而HS品种的APx在30 hpi达到最大活性，CAT和GR活性在36 hpi达到最大活性。还使用qRT-PCR评估了转录本积累情况，结果显示在1.5 mM浓度的SA引发处理后，R品种的mRNA积累量最大。
本研究证明了用SA进行种子引发处理对有效引发番茄幼苗对细菌性斑点病的防御反应具有潜在益处。
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