Department of Medicine Laboratory, Department of Cardiac Function Examination, The Second People's Hospital of Lianyungang (Cancer Hospital of Lianyungang), Lianyungang, China.
Department of Laboratory Medicine, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.
Front Cell Infect Microbiol. 2022 Apr 21;12:878813. doi: 10.3389/fcimb.2022.878813. eCollection 2022.
A recombinase polymerase amplification-lateral flow strip assay was established for detection of the outer membrane protein P6 () and the capsule encoding gene of and the detection limit, sensitivity, and specificity were determined. Specific primers and probes were designed based on the published nucleotide sequences of and . The minimum detection limit was determined with standard strains and the practical applicability of the RPA-LFS assay was assessed by detection of 209 clinical samples. The results confirmed that the RPA-LFS assay was both specific and sensitive for the detection of capsulated and non-capsulated with a detection limit of 1 CFU/µL. The detection rate of the 209 clinical samples was 97.1%, while the detection rate of capsulated was 63.2%. The detection results were consistent with the traditional culture method and dual polymerase chain reaction (PCR), confirming the applicability of the RPA-LFS assay.
建立了一种重组酶聚合酶扩增-侧向流纸条检测法，用于检测 和 的外膜蛋白 P6()和囊编码基因 ，并确定了检测限、灵敏度和特异性。根据 和 的已发表核苷酸序列设计了特异性引物和探针。用标准菌株确定了最小检测限，并通过检测 209 份临床样本评估了 RPA-LFS 检测法的实际适用性。结果证实，RPA-LFS 检测法对囊和非囊 的检测具有特异性和敏感性，检测限为 1 CFU/μL。209 份临床样本的检测率为 97.1%，而囊 的检测率为 63.2%。检测结果与传统培养方法和双重聚合酶链反应 (PCR) 一致，证实了 RPA-LFS 检测法的适用性。
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