Attia Tamer Z, Elnady Mohamed, Derayea Sayed M
Department of Analytical Chemistry, Faculty of Pharmacy, Minia University Minia Egypt
RSC Adv. 2019 Sep 23;9(51):29942-29948. doi: 10.1039/c9ra06164a. eCollection 2019 Sep 18.
A new, simple, highly sensitive and selective spectrofluorimetric method was developed for determination of gabapentin through its derivatization with -phthalaldehyde in the presence of 2-mercaptoethanol. The resulting product was highly fluorescent and its emission intensity was measured at 431 nm after excitation at 335 nm. The effect of different experimental parameters on the formation and stability of the fluorescent product was carefully studied and optimized. The fluorescence-concentration plot was rectilinear over the range of 25-125 ng mL. The lower detection and quantification limits were 3.4 mL and 11.2 ng mL, respectively. The procedure was fully validated according to the guidelines of the International Conference on Harmonization. The proposed method was successfully applied for the determination of the investigated drug in its pharmaceutical capsules and the results were in agreement with those of the reported method, in terms of the accuracy and precision. The low cost of analysis and high sensitivity make the proposed method ideally suited for analysis of the investigated drug in quality control laboratories.
开发了一种新的、简单的、高灵敏度和高选择性的荧光分光光度法，用于在2-巯基乙醇存在下通过加巴喷丁与邻苯二甲醛衍生化来测定加巴喷丁。所得产物具有高荧光性，在335nm激发后于431nm处测量其发射强度。仔细研究并优化了不同实验参数对荧光产物形成和稳定性的影响。荧光-浓度曲线在25-125 ng/mL范围内呈线性。检测限和定量限分别为3.4 ng/mL和11.2 ng/mL。该方法根据国际协调会议的指导原则进行了全面验证。所提出的方法成功应用于测定其药物胶囊中的被研究药物，结果在准确性和精密度方面与报道的方法一致。分析成本低和灵敏度高使得所提出的方法非常适合在质量控制实验室中分析被研究药物。
