Kersten P J, Kirk T K
J Bacteriol. 1987 May;169(5):2195-201. doi: 10.1128/jb.169.5.2195-2201.1987.
The importance of extracellular H2O2 in lignin degradation has become increasingly apparent with the recent discovery of H2O2-requiring ligninases produced by white-rot fungi. Here we describe a new H2O2-producing activity of Phanerochaete chrysosporium that involves extracellular oxidases able to use simple aldehyde, alpha-hydroxycarbonyl, or alpha-dicarbonyl compounds as substrates. The activity is expressed during secondary metabolism, when the ligninases are also expressed. Analytical isoelectric focusing of the extracellular proteins, followed by activity staining, indicated that minor proteins with broad substrate specificities are responsible for the oxidase activity. Two of the oxidase substrates, glyoxal and methylglyoxal, were also identified, as their quinoxaline derivatives, in the culture fluid as secondary metabolites. The significance of these findings is discussed with respect to lignin degradation and other proposed systems for H2O2 production in P. chrysosporium.
随着最近发现白腐真菌产生的需要过氧化氢的木质素酶，细胞外过氧化氢在木质素降解中的重要性日益明显。在此，我们描述了黄孢原毛平革菌一种新的产生过氧化氢的活性，该活性涉及能够使用简单醛、α - 羟基羰基或α - 二羰基化合物作为底物的细胞外氧化酶。这种活性在次生代谢过程中表达，此时木质素酶也会表达。对细胞外蛋白质进行分析等电聚焦，随后进行活性染色，结果表明具有广泛底物特异性的次要蛋白质负责氧化酶活性。两种氧化酶底物乙二醛和甲基乙二醛，也以其喹喔啉衍生物的形式，作为次生代谢产物在培养液中被鉴定出来。我们针对木质素降解以及黄孢原毛平革菌中其他提出的过氧化氢产生系统对这些发现的意义进行了讨论。
