Guo Xiao-Ying, Wang Yu-Wei, Yu Xiao, Yang Ran, Wang Ling-Na, Zhang Fang, Zhang Yong-Qing
School of Pharmacy, Shandong University of Traditional Chinese Medicine Ji'nan 250355, China.
Shandong Medicine Technician College Tai'an 271016, China.
Zhongguo Zhong Yao Za Zhi. 2022 Apr;47(8):2148-2157. doi: 10.19540/j.cnki.cjcmm.20220114.101.
This study aims to develop an HPLC-DAD method for simultaneous determination of 11 components(6 phenolic acids and 5 iridoids) in Lonicera japonica flowers(LjF) and leaves(LjL), and compare the content differences of LjF at different development stages, LjL at different maturity levels, and between LjF and LjL. One-way ANOVA, principal component analysis(PCA), and orthogonal partial least-squares discriminant analysis(OPLS-DA) were employed to compare the content of the 11 components. The content of total phenolic acids, total iridoid glycosides, and total 11 components in LjF showed an overall downward trend with the development of flowers. The content of total phenolic acids, total iridoid glycosides, and total 11 components in young leaves were higher than those in mature leaves. The results of PCA showed that the samples at different flowering stages had distinguishable differences in component content. The VIP value of OPLS-DA showed that isochlorogenic acid A, chlorogenic acid, and secologanic acid were the main differential components of LjF at different development stages or LjL with different maturity levels. LjF and LjL have certain similarities in chemical composition while significant differences in component content. The content of total phenolic acids in young leaves was significantly higher than that in LjF at various development stages. The content of total iridoid glycosides in young leaves was similar to that in LjF before white flower bud stage. The total content of 11 components in young leaves was significantly higher than that in LjF at green flower bud stage, before and during completely white flower bud stage. LjL have great potential for development. Follow-up research on the pharmacodynamic equivalence of LjF and LjL(especially young leaves) should be carried out to speed up the development and application of LjL.
本研究旨在建立一种高效液相色谱 - 二极管阵列检测法（HPLC - DAD），用于同时测定金银花花朵（LjF）和叶片（LjL）中的11种成分（6种酚酸和5种环烯醚萜），并比较不同发育阶段的LjF、不同成熟度的LjL以及LjF与LjL之间的含量差异。采用单因素方差分析、主成分分析（PCA）和正交偏最小二乘法判别分析（OPLS - DA）对11种成分的含量进行比较。LjF中总酚酸、总环烯醚萜苷和11种成分的总含量随着花朵的发育呈总体下降趋势。幼叶中总酚酸、总环烯醚萜苷和11种成分的总含量高于成熟叶。PCA结果表明，不同开花阶段的样品在成分含量上有明显差异。OPLS - DA的VIP值表明，异绿原酸A、绿原酸和裂环马钱子苷酸是不同发育阶段的LjF或不同成熟度的LjL的主要差异成分。LjF和LjL在化学成分上有一定相似性，但在成分含量上有显著差异。幼叶中总酚酸的含量显著高于各发育阶段的LjF。幼叶中总环烯醚萜苷的含量与白花蕾期之前的LjF相似。幼叶中11种成分的总含量显著高于绿蕾期、完全白花蕾期之前和期间的LjF。LjL具有很大的开发潜力。应开展LjF和LjL（尤其是幼叶）药效学等效性的后续研究，以加快LjL的开发和应用。
