Kou Yan-Nan, Zhang Xu, Kong Fu-Kang, Dong Hong-Jing, Yang Jian, Wang Wei-Hao, Kong De-Zhi, Yang Bin
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China Institute of Integrated Traditional Chinese and Western Medicine, Hebei Medical University Shijiazhuang 050017, China.
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China College of Pharmacy, Hebei Medical University Shijiazhuang 050017, China.
Zhongguo Zhong Yao Za Zhi. 2024 May;49(10):2654-2665. doi: 10.19540/j.cnki.cjcmm.20240129.101.
This study established an ultrasound-assisted extraction-high performance liquid chromatography method for simulta-neously determinining the content of 11 bioactive compounds including iridoids, phenolic acids, and flavonoids in Lonicera japonica flowers. The flowers at six stages from the rice bud stage(ML) to the golden flower stage(JH) of L. japonica varieties 'Sijuhua' and 'Beihua No.1' in two planting bases in Shandong province were collected. The established method was employed to determine the content of 11 target compounds, on the basis of which the dynamics of active components in L. japonica sampels during different development stages was investigated. The correlation analysis was carried out to reveal the correlations of the content of iridoids, phenolic acids, and flavonoids. Furthermore, the antioxidant activities of samples at different developmental stages were determined, and the relationship between antioxidant activity and chemical components was analyzed by the correlation analysis. The results showed that the total content of the 11 components in 'Sijihua' changed in a "W" pattern from the ML to JH, being the highest at the ML and the second at the slight white stage(EB). The total content of 11 compounds in 'Beihua No.1' was the highest at the ML and decreased gra-dually from the ML to JH. The samples of 'Sijihua' had higher content of iridoids and lower content of phenolic acids than those of 'Beihua No.1'. The content of flavonoids and phenolic acids showed a positive correlation(R2=0.90, P<0.05) in 'Sijihua' but no obvious correlation in 'Beihua No.1'. The antioxidant activity and phenolic acid content showed positive correlations, with the determination coefficients(R2) of 0.84(P<0.05) in 'Beihua No.1' and 0.73(P<0.05) in 'Sijihua'. The antioxidant activity of both varieties was the strongest at the ML and the second at the EB. This study revealed that the content dynamics of iridoids, phenolic acids, and flavonoids in 'Sijihua' and 'Beihua No.1' cultivated in Shandong province during different developmental stages. The results indicated that the antioxidant activity of L. japonica flowers was significantly correlated with the content of phenolic acids at different deve-lopmental stages, which provided a basis for determining the optimum harvest time of L. japonica flowers.
本研究建立了一种超声辅助萃取-高效液相色谱法，用于同时测定金银花中包括环烯醚萜类、酚酸类和黄酮类在内的11种生物活性化合物的含量。采集了山东省两个种植基地中‘四季花’和‘北花一号’金银花品种从米蕾期（ML）到金花期（JH）六个阶段的花朵。采用所建立的方法测定11种目标化合物的含量，并在此基础上研究金银花样品在不同发育阶段活性成分的动态变化。进行相关性分析以揭示环烯醚萜类、酚酸类和黄酮类含量之间的相关性。此外，测定了不同发育阶段样品的抗氧化活性，并通过相关性分析分析了抗氧化活性与化学成分之间的关系。结果表明，‘四季花’中11种成分的总含量从ML到JH呈“W”形变化，在ML期最高，在微白期（EB）次之。‘北花一号’中11种化合物的总含量在ML期最高，从ML到JH逐渐降低。‘四季花’样品中环烯醚萜类含量高于‘北花一号’，酚酸类含量低于‘北花一号’。‘四季花’中黄酮类和酚酸类含量呈正相关（R² = 0.90，P < 0.05），而‘北花一号’中无明显相关性。抗氧化活性与酚酸类含量呈正相关，‘北花一号’的决定系数（R²）为0.84（P < 0.05），‘四季花’为0.73（P < 0.05）。两个品种的抗氧化活性在ML期最强，在EB期次之。本研究揭示了山东省种植的‘四季花’和‘北花一号’金银花在不同发育阶段环烯醚萜类、酚酸类和黄酮类的含量动态变化。结果表明，金银花在不同发育阶段的抗氧化活性与酚酸类含量显著相关，这为确定金银花的最佳采收时间提供了依据。
