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与玄参科相关物种的完整叶绿体基因组比较分析。

Complete chloroplast genome of and comparative analysis with related species from Gentianaceae.

机构信息

Key Laboratory of Chinese Medicine Resources Conservation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Institute of Medicinal Plant Development, Beijing, China.

Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi, China.

出版信息

Genome. 2022 Jul 1;65(7):363-375. doi: 10.1139/gen-2021-0080. Epub 2022 May 7.

Abstract

is an essential medicinal plant in China with high ornamental and medicinal values. Unfortunately, the study of the chloroplast genome of this plant still has a gap. This study sequenced and characterized the complete chloroplast genome of . The complete chloroplast genome of is a typical circular structure of 151 123 bp. It consists of a large single-copy region (82 690 bp) and a small single-copy region (17 887 bp) separated by a pair of inverted repeats (25 273 bp), which covers 78 protein-coding genes, 30 tRNAs, and 4 rRNAs. The long repeat sequence analysis showed that the P-type (palindromic) sequences were the major long repeat sequences. Thirty-seven simple sequence repeats were identified, most of which were single nucleotides. The Bayesian inference tree, maximum likelihood tree, and neighbor-joining tree suggested that is grouped with and . The divergence time analysis showed that diverged at 1.243 Mya. Comparative analysis of chloroplast genomes can reveal interspecific diversity, and regions with high variation can be used to develop molecular markers applicable to various research areas. Our results provide a new insight into plastome evolution and a valuable resource for further studies on

摘要

是中国一种具有高观赏和药用价值的重要药用植物。不幸的是,对这种植物叶绿体基因组的研究仍存在空白。本研究对 进行了测序和特征分析,得到了其完整的叶绿体基因组。 的完整叶绿体基因组是一个典型的圆形结构,大小为 151123bp。它由一个大的单拷贝区(82690bp)和一个小的单拷贝区(17887bp)组成,这两个区域由一对反向重复序列(25273bp)隔开,其中包含 78 个蛋白编码基因、30 个 tRNA 和 4 个 rRNA。长重复序列分析表明,P 型(回文)序列是主要的长重复序列。鉴定出 37 个简单序列重复,其中大多数为单核苷酸。贝叶斯推断树、最大似然树和邻接树表明 与 和 聚为一类。分化时间分析表明,在 1.243 Mya 时发生了分化。叶绿体基因组的比较分析可以揭示种间多样性,并且高变区域可用于开发适用于各个研究领域的分子标记。我们的研究结果为质体进化提供了新的见解,并为进一步研究 提供了有价值的资源。

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