直接从阴道拭子中快速检测B族链球菌。
Rapid detection of group B streptococci directly from vaginal swabs.
作者信息
Wald E R, Dashefsky B, Green M, Harger J, Parise M, Korey C, Byers C
出版信息
J Clin Microbiol. 1987 Mar;25(3):573-4. doi: 10.1128/jcm.25.3.573-574.1987.
Abstract
Duplicate vaginal swabs were obtained from patients who attended obstetric or gynecologic clinics affiliated with the Magee Womens Hospital in Pittsburgh. One swab was cultured semiquantitatively on 5% sheep blood agar to detect group B streptococci (GBS). The other swab was subjected to a rapid method (25 min) for antigen detection and micronitrous acid exposure to extract the GBS antigen, followed by latex particle agglutination. A total of 464 swabs were evaluated by direct plating. Fifty-two swabs (11.2%) were found to contain GBS. Overall, the rapid method detected 21 of 52, or 40.4%, positive specimens. The sensitivity of the rapid method for identifying the most heavily colonized samples was 85.7%. This method can be used to identify maternity patients who are heavily colonized with GBS and are at high risk of delivering septic infants.
摘要
从匹兹堡梅吉妇女医院附属的妇产科诊所就诊的患者中获取重复的阴道拭子。一份拭子在5%羊血琼脂上进行半定量培养，以检测B族链球菌（GBS）。另一份拭子采用快速方法（25分钟）进行抗原检测，并通过亚硝酸暴露提取GBS抗原，随后进行乳胶颗粒凝集试验。通过直接接种对总共464份拭子进行了评估。发现52份拭子（11.2%）含有GBS。总体而言，快速方法检测出52份阳性标本中的21份，即40.4%。快速方法识别定植最严重样本的灵敏度为85.7%。该方法可用于识别GBS定植严重且分娩败血症婴儿风险高的孕产妇。
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