Larkin L H, Welch D E, Ogilvie S, Wubbel L
J Histochem Cytochem. 1987 Jun;35(6):693-7. doi: 10.1177/35.6.3553319.
We administered estradiol and progesterone to spayed guinea pigs, with resultant accumulation of secretory granules in endometrial gland cells. By initially employing protein A-colloidal gold immunolocalization of relaxin, followed by cytochemical staining of carbohydrate with the thiocarbohydrazide-silver proteinate method on the same section, we showed clearly that the secretory granules were composed of a central core containing relaxin and a cortex of carbohydrate-rich material. Use of normal rabbit serum rather than relaxin antiserum, and omission of periodic acid, demonstrated the specificity of the technique.
我们给切除卵巢的豚鼠注射雌二醇和孕酮，结果子宫内膜腺细胞中出现分泌颗粒的积累。通过最初采用蛋白A-胶体金免疫定位法检测松弛素，随后在同一切片上用硫代碳酰肼-蛋白银法对碳水化合物进行细胞化学染色，我们清楚地表明，分泌颗粒由含有松弛素的中央核心和富含碳水化合物的物质皮层组成。使用正常兔血清而非松弛素抗血清，以及省略高碘酸，证明了该技术的特异性。
