Study Enhanced Enzyme Diffusion with High-Speed Single Nanoparticle Rotational and Translational Tracking.

Catalytic enzymes exhibiting enhanced motion have drawn extensive attention over the past decade; nevertheless, little is known about the effect on the environment induced by enzymes. Herein, we studied the active urease system by simultaneously monitoring the diffusion of single anisotropic gold nanorods (AuNRs) with high speed dark-field imaging. We found both the translational and the rotational diffusion coefficients of AuNRs were enhanced but with inconsistent degrees, indicating the catalytic reaction had a minor effect on the physiochemical properties of the environment according to the Stokes-Einstein equation. With the increase of substrate concentration, the diffusion of AuNRs showed increased spatial but decreased temporal heterogeneity. Additionally, high speed imaging revealed AuNRs could experience intermittent ballistic motion for tens of milliseconds. These results imply inhomogeneous distribution of enzymes in free solution induced by active enzymatic reactions.