通过免疫荧光和流式细胞术对小鼠表皮细胞上的Skn抗原进行检测。

Demonstration of Skn antigens on mouse epidermal cells by immunofluorescence and flow cytometry.

作者信息

Jackman S H, Goldberg E H

出版信息

J Invest Dermatol. 1987 May;88(5):574-6. doi: 10.1111/1523-1747.ep12470180.

Abstract

C57BL/6 (B6) mice that were lethally irradiated and reconstituted with (B6XA/J)F1 spleen cells were immunized against the skin-specific antigens, Skn, by grafting with A/J tail skin. Serum from these mice was shown to contain Skna-specific antibody by a flow cytometric assay using indirect immunofluorescence.

摘要

用（B6XA/J）F1脾细胞对经致死剂量照射并重建的C57BL/6（B6）小鼠进行免疫，使其针对皮肤特异性抗原Skn产生免疫，方法是移植A/J尾皮。通过使用间接免疫荧光的流式细胞术检测表明，这些小鼠的血清中含有Skna特异性抗体。