Hamburger A W
Int J Cell Cloning. 1987 Mar;5(2):89-107. doi: 10.1002/stem.5530050202.
The human tumor stem cell (clonogenic) assay (HTCA) is a soft agar system designed for growing fresh human tumor specimens in vitro. The assay has been extensively used in studies both of individual patients' response to chemotherapy and for screening new agents. The technical limitations of this assay have been extensively discussed. The use of this test as a model system to study fundamentals of tumor cell growth has not been stressed. The potentials and limitations of this assay for the study of the regulation of tumor growth are presented.
人肿瘤干细胞（克隆形成）检测（HTCA）是一种软琼脂系统，旨在体外培养新鲜的人肿瘤标本。该检测已广泛应用于研究个体患者对化疗的反应以及筛选新药物的研究中。该检测的技术局限性已得到广泛讨论。但该检测作为研究肿瘤细胞生长基本原理的模型系统的用途尚未得到重视。本文介绍了该检测在肿瘤生长调控研究中的潜力和局限性。
