Fraysier H D
J Forensic Sci. 1987 Mar;32(2):527-30.
Phase contrast microscopy has been used for some time to search for and identify spermatozoa. An enhancement to the technique using xylene in conjuction with phase contrast microscopy is discussed. The method has been found to save time by allowing identification of spermatozoa with xylene-enhanced phase contrast microscopy in many cases that would have been unsuccessful using the normal "dry" phase contrast microscopy techniques. This eliminates a staining and reexamination step.
相差显微镜已被用于寻找和识别精子一段时间了。本文讨论了一种将二甲苯与相差显微镜结合使用的技术改进方法。已经发现,该方法通过在许多使用常规“干式”相差显微镜技术无法成功的情况下,利用二甲苯增强相差显微镜来识别精子,从而节省了时间。这省去了染色和重新检查的步骤。