Cooper Trevor G, Hellenkemper Barbara
Centre of Reproductive Medicine and Andrology, University of Münster, Domagkstrasse 11, D-48129 Münster, Germany.
J Androl. 2009 May-Jun;30(3):214-8. doi: 10.2164/jandrol.108.005819. Epub 2008 Jul 3.
Comparison of methods that estimate viability of human spermatozoa by monitoring head membrane permeability revealed that wet preparations (whether using positive or negative phase-contrast microscopy) generated significantly higher percentages of nonviable cells than did air-dried eosin-nigrosin smears. Only with the latter method did the sum of motile (presumed live) and stained (presumed dead) preparations never exceed 100%, making this the method of choice for sperm viability estimates.
通过监测头部膜通透性来估计人类精子活力的方法比较显示，湿片制备法（无论使用正相或负相相差显微镜）产生的非活细胞百分比显著高于空气干燥的伊红 - 黑色素涂片。只有使用后一种方法时，活动的（推测为活的）和染色的（推测为死的）精子总数才不会超过100%，这使得它成为精子活力评估的首选方法。
