Bakker D A, Ludwin S K
J Neurol Sci. 1987 Apr;78(2):125-37. doi: 10.1016/0022-510x(87)90055-4.
Blood vessels in the superior cerebellar peduncles were studied in Cuprizone-fed mice for leakage of proteins into the parenchyma. The status of the blood-brain barrier was determined at various stages of demyelination both by tracer methods using horseradish peroxidase and immunochemically using antisera to extravasated serum proteins and was compared to three positive and negative control conditions. The results showed no evidence of significant protein leakage into the subendothelial basement membrane or extravascular space in Cuprizone mice, during the development of demyelination. The apparent lack of damage to the blood-brain barrier found in Cuprizone animals as compared to the blood-brain barrier alterations previously reported for immune-mediated demyelinating diseases, confirms the theory that these alterations are not a non-specific association of any demyelinating process, but are of primary pathogenetic importance in immune-mediated demyelination.
研究了用双环己酮草酰二腙喂养的小鼠小脑上脚中的血管，以检测蛋白质向实质内的渗漏情况。通过使用辣根过氧化物酶的示踪方法以及使用针对外渗血清蛋白的抗血清进行免疫化学方法，在脱髓鞘的各个阶段确定血脑屏障的状态，并将其与三种阳性和阴性对照情况进行比较。结果表明，在脱髓鞘发展过程中，双环己酮草酰二腙小鼠的内皮下基底膜或血管外间隙没有明显的蛋白质渗漏迹象。与先前报道的免疫介导的脱髓鞘疾病的血脑屏障改变相比，双环己酮草酰二腙动物中血脑屏障明显未受损伤，这证实了以下理论：这些改变不是任何脱髓鞘过程的非特异性关联，而是在免疫介导的脱髓鞘中具有主要的致病重要性。
