Department of Environmental Engineering, Yeungnam University, 280 Daehak-Ro, Gyeongsan-Si, Gyeongbuk 38541, South Korea.
Department of Environmental Engineering, Yeungnam University, 280 Daehak-Ro, Gyeongsan-Si, Gyeongbuk 38541, South Korea.
J Biosci Bioeng. 2022 Jul;134(1):48-54. doi: 10.1016/j.jbiosc.2022.04.005. Epub 2022 May 6.
Operating parameters for the two-stage nitritation/anammox (PN/A) process in mainstream flows from lab-scale to pilot-scale were investigated. In the batch experiment, the specific activities of ammonia oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB) were fitted with a Monod model, and the oxygen affinity of NOB was higher than that of AOB. Nevertheless, dissolved oxygen concentrations higher than 2.0 mgO/L were effectively applied for selective inhibition of NOB. In addition, organic matter was evaluated as the operating parameter that inhibited the activity of NOB more selectively than AOB under mainstream conditions. The operating parameters determined in the batch experiment were verified in a continuous experiment, and it was shown that the shortening of hydraulic retention time with increasing aeration time in the sequencing batch reactor cycle has the potential to selectively enhance the activity of AOB over NOB. The effect on the C/N ratio and temperature in a lab-scale anammox reactor was investigated. The denitrification with the addition of organic matter contributed to the improvement of nitrogen removal efficiency, but a C/N ratio of more than 1.0 severely deteriorated the sludge settleability. The operating factors for the mainstream PN/A determined in the lab scale were applied to the pilot plant. As a result, a nitrogen removal efficiency of 79 ± 11% was stably achieved with effluent total nitrogen of 2.6 ± 1.5 mg/L. However, unpredictable fluctuations in environmental factors revealed that residual ammonium was another critical factor affecting mainstream PN/A.
考察了从实验室规模到中试规模主流流中二阶段硝化/厌氧氨氧化（PN/A）工艺的操作参数。在批处理实验中，氨氧化菌（AOB）和亚硝酸盐氧化菌（NOB）的比活性符合 Monod 模型，并且 NOB 的氧亲和力高于 AOB。然而，高于 2.0mgO/L 的溶解氧浓度可有效用于选择性抑制 NOB。此外，有机物被评估为在主流条件下比 AOB 更有选择性地抑制 NOB 活性的操作参数。在连续实验中验证了批处理实验中确定的操作参数，并表明在序批式反应器周期中增加曝气时间缩短水力停留时间有潜力选择性地增强 AOB 的活性而抑制 NOB。还考察了实验室规模厌氧氨氧化反应器中 C/N 比和温度的影响。添加有机物的反硝化有助于提高氮去除效率，但 C/N 比大于 1.0 会严重恶化污泥沉降性能。在实验室规模下确定的主流 PN/A 的操作因素被应用于中试工厂。结果，稳定实现了 79±11%的氮去除效率，出水总氮为 2.6±1.5mg/L。然而，环境因素的不可预测波动表明，残余氨是影响主流 PN/A 的另一个关键因素。
