Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, New Delhi, 110016, India.
DBT-National Centre for Cell Science, Pune, Maharashtra, 411007, India.
Arch Microbiol. 2022 May 10;204(6):308. doi: 10.1007/s00203-022-02921-x.
Fabrics serve as fomites in spreading nosocomial infections. As a patient is in close contact with bedsheets, it is important to assess the seasonal variation in bacterial diversity on these in healthcare units. The study was conducted to characterise the bacterial diversity on patients' bedsheets across 7 months in a primary healthcare unit. Polyester-cotton blend fabric was stitched on bedsheets, and temporal dynamics of bacterial communities was assessed from May to November 2019. qPCR and amplicon sequencing of 16S rRNA gene was performed for profiling of bacterial community. Results revealed the dominance of Bacillota followed by Pseudomonadota, and Actinomycetota. A seasonal variation was observed in the bacterial load, with maximum values in June. This indicates the impact of environmental conditions on bacterial abundance and composition on fabrics in healthcare unit. The presence of priority pathogens on the patient bedsheets is a human health concern reiterating the need for season-specific laundering protocol.
织物作为传播医院感染的媒介物。由于患者与床单密切接触，因此评估医疗机构中这些床单上细菌多样性的季节性变化非常重要。本研究旨在描述在基层医疗机构中 7 个月内患者床单上的细菌多样性。在床单上缝合了聚酯棉混纺面料，并于 2019 年 5 月至 11 月评估细菌群落的时间动态。进行了 16S rRNA 基因的 qPCR 和扩增子测序，以对细菌群落进行分析。结果表明，厚壁菌门占主导地位，其次是假单胞菌门和放线菌门。观察到细菌负荷存在季节性变化，6 月份达到最大值。这表明环境条件对医疗机构中织物上细菌丰度和组成的影响。在患者的床单上存在优先病原体，这是对人类健康的关注，再次强调了针对特定季节的洗涤方案的必要性。
