Key Laboratory of Marine Hazards Forecasting, Ministry of Natural Resources, Hohai University, Nanjing, 210098, PR China; College of Oceanography, Hohai University, Nanjing, 210098, PR China; College of Environment, Hohai University, Nanjing, 210098, PR China.
College of Environment, Hohai University, Nanjing, 210098, PR China.
Chemosphere. 2021 Jun;272:129855. doi: 10.1016/j.chemosphere.2021.129855. Epub 2021 Feb 13.
The microbial community composition in aquatic ecosystems have received increased attention. However, the knowledge gap relative to the responses of bacterial, archaeal and fungal communities in co-contaminated river sediments remain poorly studied. Here, we investigated the changes of tetrabromobisphenol A (TBBPA) and copper (Cu) concentrations and the responses of microbial communities in co-contaminated sediments during long-term incubation. TBBPA concentrations significantly decreased over time, whereas Cu concentrations remained relatively stable over the 60-day incubation. Abundances of the bacterial 16S rRNA, archaeal 16S rRNA and fungal ITS genes ranged from 6.53 × 10 to 1.26 × 10 copies g, 1.12 × 10 to 5.47 × 10 copies g and 5.33 × 10 to 7.51 × 10 copies g in the samples, respectively. A total of 11, 6 and 5 bacterial, archaeal and fungal phyla were identified across all samples. Bacterial, archaeal and fungal communities mainly consisted of members from the phyla Proteobacteria and Acidobacteria, Methanomicrobia and Woesearchaeia as well as Agaricales and Helotiales, respectively. Fungal communities showed a stronger response to pollutant addition after a long incubation compared with bacterial and archaeal communities. The variance analysis results revealed that the bacterial, archaeal and fungal microbial communities of all treatments were distinctly distributed into two separated clusters according to incubation time. However, the three microbial communities did not significantly change in response to pollutant types, which was consistent with variation in relative abundances of the three microbial communities. These findings improve our understanding of the ecotoxicological effects of co-exposure on sediment microbial communities.
水生生态系统中的微生物群落组成受到了越来越多的关注。然而，有关细菌、古菌和真菌群落对共污染河底沉积物的响应的知识差距仍然研究甚少。在这里，我们研究了在长期孵育过程中，四溴双酚 A (TBBPA)和铜 (Cu)浓度的变化以及共污染沉积物中微生物群落的响应。TBBPA 浓度随时间显著降低，而 Cu 浓度在 60 天的孵育过程中相对稳定。细菌 16S rRNA、古菌 16S rRNA 和真菌 ITS 基因的丰度分别为 6.53×10 至 1.26×10 拷贝 g-1、1.12×10 至 5.47×10 拷贝 g-1 和 5.33×10 至 7.51×10 拷贝 g-1。在所有样品中，共鉴定出 11、6 和 5 个细菌、古菌和真菌门。细菌、古菌和真菌群落主要由变形菌门和酸杆菌门、甲烷微菌门和沃氏古菌门以及伞菌纲和鹅膏菌纲的成员组成。与细菌和古菌群落相比，真菌群落经过长时间孵育后对污染物添加的反应更强。方差分析结果表明，根据孵育时间，所有处理的细菌、古菌和真菌微生物群落明显分为两个分离的聚类。然而，三种微生物群落对污染物类型的变化没有显著响应，这与三种微生物群落相对丰度的变化一致。这些发现提高了我们对共暴露对沉积物微生物群落生态毒理学影响的理解。
