Cardio-vascular Surgery, Ningbo First Hospital, Ningbo, China.
J Clin Lab Anal. 2022 Jun;36(6):e24487. doi: 10.1002/jcla.24487. Epub 2022 May 9.
The diagnosis and treatment of rheumatic valvular heart disease (RVHD) require substantial improvements. Studies found that circular RNAs (circRNAs) are involved in the progression of cardiovascular diseases. We screened target hsa_circ_0003748 by circRNA microarrays uploaded to a database. We used fluorescence in situ hybridization to determine the cellular location of hsa_circ_0003748. A dual-luciferase reporter gene assay revealed that has_circ_0003748 might bind the miRNA miR-577. In hVIC cells (an RVHD cell line), Cell Counting Kit-8, Transwell, and flow cytometry assays measured proliferation, migration, and cell cycle and apoptosis, respectively. We found that hsa_circ_0003748 was localized in the cytoplasm; hsa_circ_0003748 promoted the proliferation and migration of hVIC cells, arrested the cell cycle in the G2/M phase, and inhibited apoptosis. These phenomena may result from hsa_circ_0003748 promoting RVHD after sponging miR-577. Bioinformatic analysis revealed that hsa_circ_0003748 might affect RVHD progression by affecting transcription and the MAPK signaling pathway, the Ras signaling pathway, the cAMP signaling pathway, the Rap1 signaling pathway, and other signaling pathways.
风湿性心脏瓣膜病（RVHD）的诊断和治疗需要显著改善。研究发现，环状 RNA（circRNAs）参与了心血管疾病的进展。我们通过上传到数据库的 circRNA 微阵列筛选了靶 hsa_circ_0003748。我们使用荧光原位杂交来确定 hsa_circ_0003748 的细胞位置。双荧光素酶报告基因检测显示 hsa_circ_0003748 可能与 miRNA miR-577 结合。在 hVIC 细胞（RVHD 细胞系）中，通过细胞计数试剂盒-8、Transwell 和流式细胞术分别测量增殖、迁移和细胞周期以及细胞凋亡。我们发现 hsa_circ_0003748 定位于细胞质中；hsa_circ_0003748 促进 hVIC 细胞的增殖和迁移，将细胞周期阻滞在 G2/M 期，并抑制细胞凋亡。这些现象可能是由于 hsa_circ_0003748 通过海绵吸附 miR-577 促进 RVHD 所致。生物信息学分析表明，hsa_circ_0003748 可能通过影响转录和 MAPK 信号通路、Ras 信号通路、cAMP 信号通路、Rap1 信号通路和其他信号通路来影响 RVHD 的进展。
