Hsa_circ_0000437 上调并促进风湿性心脏瓣膜病的疾病进展。
Hsa_circ_0000437 upregulates and promotes disease progression in rheumatic valvular heart disease.
机构信息
Department of Cardiothoracic Surgery, Ningbo Medical Centre Lihuili Hospital, Ningbo University, Ningbo, China.
Medical School of Ningbo University, Ningbo, China.
出版信息
J Clin Lab Anal. 2022 Feb;36(2):e24197. doi: 10.1002/jcla.24197. Epub 2021 Dec 24.
BACKGROUND
Currently, the diagnosis and outcome of rheumatic valvular heart disease (RVHD) are less than ideal, and there are no accurate biomarkers. Circular RNA (circRNA) might participate in the occurrence and development of RVHD.
MATERIALS AND METHODS
We use circRNA microarray to filter out the target has_circ_0000437. qRT-PCR was used to measure the expression levels of hsa_circ_0000437 in RVHD plasma samples. We assessed the diagnostic value of hsa_circ_0000437 in RVHD. Cell function in vitro experiment was to explore the effect of has_circ_0000437 on RVHD.
RESULTS
Has_circ_0000437 is highly expressed in RVHD (p < 0.001). has_circ_0000437 has the diagnostic value in RVHD. In RVHD, hsa_circ_0000437 can promote cell proliferation and migration but inhibits its apoptosis. This may be due to the combination of has_circ_0000437 and target miRNA in the cytoplasm that affects the progress of RVHD.
CONCLUSIONS
Has_circ_0000437 can promote the process of RVHD and may be a potential for the diagnosis and treatment of RVHD.
背景
目前，风湿性心脏瓣膜病（RVHD）的诊断和预后并不理想，且缺乏准确的生物标志物。环状 RNA（circRNA）可能参与 RVHD 的发生和发展。
材料和方法
我们使用 circRNA 微阵列筛选出目标 hsa_circ_0000437。qRT-PCR 用于测量 RVHD 血浆样本中 hsa_circ_0000437 的表达水平。我们评估了 hsa_circ_0000437 在 RVHD 中的诊断价值。体外细胞功能实验旨在探讨 hsa_circ_0000437 对 RVHD 的影响。
结果
hsa_circ_0000437 在 RVHD 中高表达（p<0.001）。hsa_circ_0000437 对 RVHD 具有诊断价值。在 RVHD 中，hsa_circ_0000437 可以促进细胞增殖和迁移，但抑制其凋亡。这可能是由于 hsa_circ_0000437 与细胞质中的靶 miRNA 结合，影响 RVHD 的进展。
结论
hsa_circ_0000437 可以促进 RVHD 的进程，可能是 RVHD 诊断和治疗的潜在靶点。
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