Department of Preventive and Restorative Dentistry, São Paulo State University (Unesp), School of Dentistry, Araçatuba, SP, Brazil.
Department of Chemistry, Federal University of São Carlos (UFSCar), São Carlos, SP, Brazil.
Biofouling. 2022 Apr;38(4):321-330. doi: 10.1080/08927014.2022.2064220. Epub 2022 May 10.
This study evaluated the effects of micrometric or nano-sized sodium hexametaphosphate (HMPnano), combined or not with fluoride (NaF, 1100 ppm), on dual-species biofilms of and . Biofilms were treated with solutions containing the polyphosphates at 0.5% or 1.0%, with/without fluoride (F), in addition to positive and negative controls. Biofilms were analysed by colony-forming units (CFU) counting, metabolic activity, production of biomass, composition of extracellular matrix, and structure. 1% HMPnano + F led to the lowest CFU, while CFU counts were not affected by any solution. 1% HMPnano led to the lowest metabolic activity, except for 1% HMPnano + F. All solutions promoted reductions in biofilm biomass compared to controls. Also, 1% HMPnano + F promoted the lowest concentrations of carbohydrates in the biofilm matrix, besides substantially affecting biofilms' structure. In conclusion, HMPnano and F promoted higher antibiofilm effects compared with its micrometric counterpart for most of the parameters assessed.
本研究评估了微米或纳米级六偏磷酸钠（HMPnano）与氟化物（NaF，1100ppm）联合或不联合对 和 双物种生物膜的影响。生物膜用含有多磷酸盐的溶液处理，浓度为 0.5%或 1.0%，有/无氟化物（F），并设有阳性和阴性对照。通过集落形成单位（CFU）计数、代谢活性、生物量产生、细胞外基质组成和结构分析生物膜。1% HMPnano+F 导致最低的 CFU，而任何溶液都不会影响 CFU 计数。1% HMPnano 导致最低的代谢活性，除了 1% HMPnano+F。与对照组相比，所有溶液均降低了生物膜的生物量。此外，1% HMPnano+F 还促进了生物膜基质中最低浓度的碳水化合物，同时还显著影响了生物膜的结构。总之，与微米级的六偏磷酸钠相比，HMPnano 和 F 对大多数评估参数都表现出更高的抗生物膜作用。
