Department of Pediatric Dentistry and Public Health, São Paulo State University (Unesp), School of Dentistry Araçatuba, SP, Brazil.
Graduate Program in Dentistry, University of Western São Paulo (UNOESTE), Presidente Prudente, SP, Brazil.
Biofouling. 2019 Jul;35(6):710-718. doi: 10.1080/08927014.2019.1653455. Epub 2019 Aug 27.
This study aimed to evaluate the effect of sodium trimetaphosphate (TMP), either with fluoride (F) or without fluoride, on dual-species biofilms of and . The 72 h biofilms were treated with 0.25%, 0.5%, or 1% TMP solutions, combined or not with 500 ppm F, and analysed by quantification of viable plate counts, metabolic activity, biomass, and extracellular matrix components. Biofilm structure was evaluated by scanning electron microscopy (SEM). TMP significantly reduced the number of cells and biomass only when associated to F. Furthermore, fluoride-free TMP promoted significant reductions in biofilm metabolism, while all the tested solutions decreased the contents of the biofilm matrix compared to untreated groups. Regarding biofilm structure, TMP associated with F led to the formation of a less compact biofilm. It was concluded that TMP alone had a reducing effect, mainly on the metabolism and the extracellular matrix components of the biofilms.
本研究旨在评估三聚磷酸钠（TMP）在与氟化物（F）结合或不结合的情况下对 和 的双物种生物膜的影响。用 0.25%、0.5%或 1%的 TMP 溶液处理 72 小时生物膜，结合或不结合 500ppm 的 F，并通过活菌平板计数、代谢活性、生物量和细胞外基质成分的定量分析来分析。通过扫描电子显微镜（SEM）评估生物膜结构。只有当与 F 结合时，TMP 才能显著减少 细胞数量和生物量。此外，不含氟的 TMP 可显著降低生物膜代谢，而所有测试溶液与未处理组相比均降低了生物膜基质的含量。关于生物膜结构，与 F 结合的 TMP 导致生物膜形成不那么紧密。研究得出结论，单独的 TMP 主要对生物膜的代谢和细胞外基质成分具有减少作用。
