College of Chemistry, Sichuan University, Chengdu 610064, P. R. China.
Institute of New Energy and Low-Carbon Technology, Sichuan University, Chengdu 610064, P. R. China.
Analyst. 2022 May 30;147(11):2361-2368. doi: 10.1039/d2an00488g.
The Rayleigh scattering method is a rising analytical technique because of its high sensitivity and simple operability in the detection of scattered particles. Herein, an efficient and facile physical shading method (covering the cuvette) based on the Rayleigh scattering pattern was developed, which exhibited overall promotion of sensitivity, linearity, detection limit, precision and accuracy because of its greatly reduced background noise upon measurement even if complicated chemical reagents and instruments were not involved. Protein, as a common scattering particle, is a direct or indirect expression of an organism's state of life. Thus, considering the simple and cost-effective features of the shading treatment, a combination strategy based on the probe was adapted to further enhance the performance of protein detection (the limit of detection (3) was reduced by at least 20 times, using Eriochrome Black T as a probe), and the possible reasons for the improvement were deduced theoretically. Furthermore, the technology was successfully applied to detect protein in human urine, giving consistent results. In general, this improvement strategy can be handily integrated into most detection systems based on Rayleigh scattering, and its realization delineates a blueprint for the rapid development of sensors.
瑞利散射法是一种新兴的分析技术，因为其在散射粒子检测方面具有高灵敏度和简单可操作性。本文基于瑞利散射模式开发了一种高效、简便的物理遮光方法（覆盖比色皿），由于测量时背景噪声大大降低，即使不使用复杂的化学试剂和仪器，也能整体提高灵敏度、线性度、检测限、精密度和准确度。蛋白质作为常见的散射粒子，是生物体生命状态的直接或间接表达。因此，考虑到遮光处理的简单和经济高效的特点，采用了基于探针的组合策略来进一步增强蛋白质检测的性能（使用 Eriochrome Black T 作为探针时，检测限（3）降低了至少 20 倍），并从理论上推断了改进的可能原因。此外，该技术成功应用于检测人尿中的蛋白质，结果一致。总的来说，这种改进策略可以方便地集成到大多数基于瑞利散射的检测系统中，其实现为传感器的快速发展描绘了蓝图。
