Lopez-Garrido J, Galera-Davidson H, Ortega Medina I, Martinez Navarro A E, Leal Bermudez J, Rodriguez Fernandez A
Lab Invest. 1987 May;56(5):534-43.
We have developed a new model of disseminated intravascular coagulation in rats based on the induction of immunosuppression by prolonged high-dose dexamethasone treatment. Most models of disseminated intravascular coagulation are based on the generalized Shwartzman reaction, which is observed characteristically in experimental animals after two separate inoculations of bacterial endotoxins. These produce massive deposition of thrombi in the microcirculation and significant hemorrhagic and ischemic phenomena. We have demonstrated that the administration of glucocorticosteroids at the specific doses and intervals can adequately replace the first (preparatory) injection of endotoxin. For this reason, we have attempted to experimentally simulate a frequent clinical situation, such as sepsis secondary to peritonitis, by intraperitoneal inoculation of Escherichia coli and hog gastric mucin into rats pretreated with dexamethasone. This inoculation was equivalent to the second injection of endotoxin in the Shwartzman model (triggering inoculation). A typical picture of disseminated intravascular coagulation induced by bacterial endotoxins developed, as demonstrated by the anatomopathologic, microbiologic, and hematologic studies performed. These results were then compared to those obtained in rats treated exclusively with dexamethasone or given, in addition, an effective antibiotic therapy.
我们基于长期大剂量地塞米松治疗诱导免疫抑制，开发了一种新的大鼠弥散性血管内凝血模型。大多数弥散性血管内凝血模型基于全身性施瓦茨曼反应，该反应的特征是在实验动物经过两次单独接种细菌内毒素后出现。这些会在微循环中产生大量血栓沉积以及显著的出血和缺血现象。我们已经证明，以特定剂量和间隔给予糖皮质激素可以充分替代首次（预备性）内毒素注射。因此，我们试图通过向用糖皮质激素预处理的大鼠腹腔内接种大肠杆菌和猪胃粘蛋白，来实验模拟一种常见的临床情况，如腹膜炎继发的败血症。这种接种相当于施瓦茨曼模型中的第二次内毒素注射（触发接种）。解剖病理学、微生物学和血液学研究表明，出现了由细菌内毒素诱导的弥散性血管内凝血的典型表现。然后将这些结果与仅用糖皮质激素治疗或另外给予有效抗生素治疗的大鼠所获得的结果进行比较。
