Dettori G, Grillo R, Agliano A M, Branca G
Microbiologica. 1987 Jan;10(1):1-8.
The antigenic cross-reactivity between intestinal treponemes of human and swine origin (T. hyodysenteriae and T. innocens) was studied by sodium-dodecylsulfate-polyacrylamide gel electrophoresis and western blotting techniques. Several distinct peptides, ranging in molecular weight from 14,400 to about 150,000 daltons, were recognized by SDS-PAGE analysis of twelve human and two swine hemolytic intestinal treponemes. Thirty antigenic bands were develope by homologous antiserum against treponeme D87, one of 12 human intestinal hemolytic treponemes isolated in our laboratory. An identical number of cross-reacting antigens was found when Western blots of human treponemes D87 were tested with antisera against ten other human treponemes or with antisera against swine treponemes (T. hyodysenteriae, T. innocens). Similar results were obtained when Western blots of swine T. hyodysenteriae were tested for cross-reactivity with antisera against all human intestinal treponemes.
采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和蛋白质印迹技术，研究了人和猪源肠道密螺旋体（猪痢疾密螺旋体和无害密螺旋体）之间的抗原交叉反应性。通过对12株人源和2株猪源溶血性肠道密螺旋体进行SDS-PAGE分析，识别出了几种不同的肽段，其分子量范围为14,400至约150,000道尔顿。用针对密螺旋体D87（我们实验室分离的12株人源肠道溶血性密螺旋体之一）的同源抗血清，显影出30条抗原带。当用人密螺旋体D87的蛋白质印迹与针对其他10种人密螺旋体的抗血清或针对猪密螺旋体（猪痢疾密螺旋体、无害密螺旋体）的抗血清进行检测时，发现了相同数量的交叉反应抗原。当用针对所有人源肠道密螺旋体的抗血清检测猪痢疾密螺旋体的蛋白质印迹的交叉反应性时，也获得了类似的结果。
