Baker-Zander S A, Lukehart S A
Infect Immun. 1984 Oct;46(1):116-21. doi: 10.1128/iai.46.1.116-121.1984.
The antigenic cross-reactivity between Treponema pallidum and several pathogenic members of the family Spirochaetaceae was examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting techniques. Blots of T. pallidum antigens were incubated with antiserum from rabbits infected or immunized with T. pallidum, Treponema paraluiscuniculi, Treponema hyodysenteriae (strains B204 and T22), Borrelia hermsii serotype 7, or Leptopsira interrogans serogroup Canicola. T. pallidum contained 22 antigenic molecules ranging from 85,000 to 12,000 daltons which were recognized by serum from rabbits infected with T. pallidum. Serum from rabbits infected with T. paraluiscuniculi cross-reacted with 21 of these molecules and faintly reacted with a band at 15,000 daltons which was not recognized by anti-T. pallidum serum. Antisera directed against strains B204 and T22 of T. hyodysenteriae cross-reacted with 11 and 10 antigens of T. pallidum, respectively. B. hermsii and L. interrogans serogroup Canicola antisera detected 11 and 10 treponemal antigens, respectively. Many of the T. pallidum antigens detected by antisera against T. hyodysenteriae, B. hermsii, or L. interrogans serogroup Canicola have been previously identified as containing moieties also found on the nonpathogenic Treponema phagedenis, biotype Reiter, and may therefore represent group antigens common to members of the family Spirochaetaceae.
通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和蛋白质印迹技术，检测了梅毒螺旋体与螺旋体科几种致病成员之间的抗原交叉反应性。将梅毒螺旋体抗原印迹与感染或免疫了梅毒螺旋体、兔梅毒螺旋体、猪痢疾短螺旋体（菌株B204和T22）、赫氏疏螺旋体血清型7或犬钩端螺旋体血清群Canicola的兔抗血清一起孵育。梅毒螺旋体含有22种抗原分子，分子量在85,000至12,000道尔顿之间，可被感染梅毒螺旋体的兔血清识别。感染兔梅毒螺旋体的兔血清与其中21种分子发生交叉反应，并与一条15,000道尔顿的条带微弱反应，该条带不被抗梅毒螺旋体血清识别。针对猪痢疾短螺旋体菌株B204和T22的抗血清分别与梅毒螺旋体的11种和10种抗原发生交叉反应。赫氏疏螺旋体和犬钩端螺旋体血清群Canicola抗血清分别检测到11种和一种梅毒螺旋体抗原。针对猪痢疾短螺旋体、赫氏疏螺旋体或犬钩端螺旋体血清群Canicola的抗血清检测到的许多梅毒螺旋体抗原先前已被鉴定为含有在非致病性细弱螺旋体生物型Reiter上也发现的部分，因此可能代表螺旋体科成员共有的群抗原。
