İstanbul University, İstanbul Faculty of Medicine, Department of Histology and Embryology, İstanbul, Turkey.
İstanbul University, Graduate School of Health Sciences, İstanbul, Turkey.
Exp Dermatol. 2022 Sep;31(9):1355-1363. doi: 10.1111/exd.14603. Epub 2022 May 15.
Vitiligo is a depigmentation disease which affects skin and hair follicles with a prevalence of 0.5%-1% worldwide. In this study, we aimed to investigate treatmental potential of dermis-derived cells in monobenzone (MBEH)-induced mouse vitiligo model with light and electron microscopy. MBEH (40%) cream was topically applied to C57BL/6 mice until depigmentation occured in vitiligo and experimental groups. In experimental groups, dermis-derived cells obtained from back skin biopsy samples before induction of vitiligo, were injected intradermally to vitiligo mice. On Days 3 and 15 after cell transplantation to experimental groups, skin biopsies were compared with biopsies of control and vitiligo groups. Dermis-derived cells obtained from back skin biopsy samples of experimental groups showed nestin and versican immunoreactivity. Melanin in hair follicles of control group was detected by histochemical stainings (Haematoxylin and eosin and Fontana-Masson) whereas sparse melanin granules were observed in hair follicles of vitiligo group. In experimental groups, there was an increase in the number of hair follicles with melanin compared with vitiligo group. We observed MART-1 immunoreactive cells mostly around the hair follicles in control group and within dermis in vitiligo group. Electron microscopic investigation showed presence of melanosomes in hair follicles of control group and lacking in vitiligo group. In experimental groups, both type of hair follicles were observed with electron microscope. Our data suggest that autologously transplanted dermis-derived cells may be effective in vitiligo treatment by contrubuting to melanin production.
白癜风是一种影响皮肤和毛囊的脱色疾病，全球患病率为 0.5%-1%。在这项研究中，我们旨在通过光镜和电镜研究真皮来源细胞在单苯氧乙醇（MBEH）诱导的小鼠白癜风模型中的治疗潜力。将 MBEH（40%）乳膏局部涂抹于 C57BL/6 小鼠，直至白癜风发生和实验组成员出现脱色。在实验组中，在诱导白癜风之前从背部皮肤活检样本中获得真皮来源细胞，并将其注射到白癜风小鼠的真皮内。在细胞移植到实验组后的第 3 天和第 15 天，将皮肤活检与对照组和白癜风组的活检进行比较。实验组从背部皮肤活检样本中获得的真皮来源细胞显示巢蛋白和 versican 免疫反应性。通过组织化学染色（苏木精和伊红和 Fontana-Masson）检测对照组毛囊中的黑色素，而白癜风组的毛囊中观察到稀疏的黑色素颗粒。在实验组中，与白癜风组相比，有黑色素的毛囊数量增加。我们在对照组中观察到大多数围绕毛囊的 MART-1 免疫反应性细胞，而在白癜风组中则在真皮内观察到。电镜检查显示对照组毛囊中有黑色素体，而白癜风组中则没有。在实验组中，两种类型的毛囊都可以用电子显微镜观察到。我们的数据表明，自体移植的真皮来源细胞可能通过促进黑色素生成而有效治疗白癜风。
