Moreira C G, Carrenho L Z B, Pawloski P L, Soley B S, Cabrini D A, Otuki M F
Department of Pharmacology, Universidade Federal do Paraná, Curitiba, PR, Brazil.
Department of Pharmaceutical sciences, Universidade Federal do Paraná, Curitiba, PR, Brazil.
J Ethnopharmacol. 2015 Jun 20;168:315-25. doi: 10.1016/j.jep.2015.03.080. Epub 2015 Apr 8.
Leaves of Pyrostegia venusta are popularly used to treat vitiligo; however, none in vivo study showed its ability.
The overall objective of the present study was to evaluate the antiinflammatory and hyperpigmentant activities of hydroethanolic (HE) extract of leaves from P. venusta in animal models of vitiligo induced by croton oil and monobenzone.
The antiinflamatory and antioxidative effects of topical and oral administration of HE extract of P. venusta were evaluated in Swiss mice on edema model induced by croton oil, and further the N-acetyl-b-d-glucosaminidase (NAG) activity, cell infiltration, and cytokine and reactive species oxygen (ROS) levels. The involvement on mice pigmentation, cell infiltration and cytokine levels were evaluated on vitiligo model induced by monobenzone in C56BL/6 mice.
HE of P. venusta by gavage (300 mg/kg) reduced NAG activity in 32.5 ± 5% on mouse ear edema induced by croton oil. Similarly, cell infiltration was lower (42.3 ± 5.9%) when compared to control group, as well as interleukin-1β (IL-1β), interleukin (IL-6) and tumor necrosis factor-α (TNF-α) levels, in 44.1 ± 9.7%, 71.9 ± 22.2% and to basal levels, respectively. Topical treatment with HE of P. venusta (10%) diminished cell infiltration (67.7 ± 7.1%) and ROS levels (total reduction). P. venusta either by gavage (300 mg/Kg) or topically (10%) increased epidermal melanin level (116.5 ± 13% and 100 ± 16.9%, respectively), diminished dermal depigmentation (36.0 ± 6.7% and 38.2 ± 6.2%, respectively), as well as tissue TNF-α levels (68.2 ± 11.6% and 99.2 ± 12.1%, respectively) and cell infiltration (basal levels and 94.3 ± 9.17%, respectively). However, only topical treatment with HE of P. venusta altered melanin specific marker in hair follicles.
For the first time these data show that topical and oral administrations of P. venusta have significant antiinflammatory and hyperpigmentant effects, demonstrating different topical and systemic effects through two animal models. Together these models are capable to mimic several features founded in vitiligo, and the results support the ethnopharmacological use of P. venusta.
连理藤的叶子在民间常用于治疗白癜风；然而，尚无体内研究表明其具有此功效。
本研究的总体目标是在巴豆油和单苯甲醚诱导的白癜风动物模型中，评估连理藤叶乙醇提取物（HE）的抗炎和色素沉着活性。
在瑞士小鼠的巴豆油诱导水肿模型中，评估连理藤叶HE提取物局部和口服给药的抗炎和抗氧化作用，并进一步检测N - 乙酰 - β - D - 氨基葡萄糖苷酶（NAG）活性、细胞浸润、细胞因子和活性氧（ROS）水平。在C56BL/6小鼠的单苯甲醚诱导的白癜风模型中，评估其对小鼠色素沉着、细胞浸润和细胞因子水平的影响。
通过灌胃给予连理藤叶HE提取物（300mg/kg）可使巴豆油诱导的小鼠耳部水肿模型中的NAG活性降低32.5±5%。同样，与对照组相比，细胞浸润较低（42.3±5.9%），白细胞介素 - 1β（IL - 1β）、白细胞介素（IL - 6）和肿瘤坏死因子 - α（TNF - α）水平分别降低44.1±9.7%、71.9±22.2%至基础水平。用连理藤叶HE提取物（10%）局部治疗可减少细胞浸润（67.7±7.1%）和ROS水平（完全降低）。连理藤叶HE提取物通过灌胃（300mg/Kg）或局部给药（10%）均可增加表皮黑色素水平（分别为116.5±13%和100±16.9%），减少真皮色素脱失（分别为36.0±6.7%和38.2±6.2%），以及组织TNF - α水平（分别为68.2±11.6%和99.2±12.1%）和细胞浸润（分别为基础水平和94.3±9.17%）。然而，只有用连理藤叶HE提取物局部治疗可改变毛囊中的黑色素特异性标记物。
这些数据首次表明，连理藤叶的局部和口服给药具有显著的抗炎和色素沉着作用，通过两种动物模型证明了不同的局部和全身作用。这些模型共同能够模拟白癜风中发现的几个特征，结果支持连理藤叶的民族药理学应用。
