Department of Cardiology and Vascular Medicine, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo General Hospital, Surabaya, Indonesia.
Cardiovasc Hematol Agents Med Chem. 2023;21(3):156-166. doi: 10.2174/1871525720666220510211116.
Several studies have shown that adipose-derived mesenchymal stem cells (AMSCs) can differentiate into mesenchymal lineages, including cardiac cell types, but complete differentiation into cardiomyocytes is challenging. Unfortunately, the optimal method to maximize AMSCs differentiation has not yet been established. Platelet-rich plasma (PRP), which contains rich growth factors, is believed to stimulate stem cell proliferation and differentiation in the context of cardiac tissue regeneration.
This study aimed to analyze the effect of PRP administration to enhance the differentiation of AMSCs into cardiomyocytes.
This study used a randomized post-test-only controlled group design. AMSCs were isolated from adipose tissues and cultured for 4 passages. The samples were divided into 3 groups, a negative control group (α-MEM), a positive control group (differentiation medium), and a treatment group (PRP). The assessment of GATA-4 expression was conducted using flow cytometry on day-5. The assessment of troponin expression was conducted using immunocytochemistry on day- 10. Data analysis was conducted using T-test and One-Way ANOVA.
Flowcytometry of GATA-4 expression revealed a significant improvement in PRP group compared to negative and positive control group (67.04 ± 4.49 vs. 58.15 ± 1.23 p < 0.05; 67.04 ± 4.49 vs. 52.96 ± 2.02 p < 0.05). This was supported by the results of immunocytochemistry on troponin expression, which revealed significant improvement in the PRP group compared to negative and positive controls (38.13 ± 5.2 vs. 10.73 ± 2.39 p < 0.05; 38.13 ± 5.2 vs. 26.00 ± 0.4 p < 0.05).
PRP administration in the AMSCs culture could significantly improve the differentiation of AMSCs into cardiomyocytes measured by GATA-4 and troponin expressions. This was concordant with our hypothesis, which stated that there was an effect of PRP administration on AMSCs differentiation into cardiomyocytes.
多项研究表明脂肪来源的间充质干细胞（AMSCs）可分化为间充质谱系，包括心肌细胞类型，但完全分化为心肌细胞具有挑战性。不幸的是，尚未确定将 AMSC 最大程度分化的最佳方法。富含血小板的血浆（PRP）富含生长因子，据信可在心脏组织再生的情况下刺激干细胞增殖和分化。
本研究旨在分析 PRP 给药对增强 AMSC 向心肌细胞分化的作用。
本研究采用随机后测试仅对照分组设计。从脂肪组织中分离出 AMSCs 并培养 4 代。将样本分为 3 组，阴性对照组（α-MEM）、阳性对照组（分化培养基）和治疗组（PRP）。第 5 天使用流式细胞术评估 GATA-4 表达。第 10 天使用免疫细胞化学评估肌钙蛋白表达。数据分析采用 T 检验和单因素方差分析。
GATA-4 表达的流式细胞术显示，PRP 组与阴性和阳性对照组相比，表达显著改善（67.04 ± 4.49 比 58.15 ± 1.23 p < 0.05；67.04 ± 4.49 比 52.96 ± 2.02 p < 0.05）。免疫细胞化学检测肌钙蛋白表达的结果也支持这一结果，与阴性和阳性对照组相比，PRP 组显著改善（38.13 ± 5.2 比 10.73 ± 2.39 p < 0.05；38.13 ± 5.2 比 26.00 ± 0.4 p < 0.05）。
在 AMSCs 培养中添加 PRP 可显著改善 GATA-4 和肌钙蛋白表达测量的 AMSCs 向心肌细胞的分化。这与我们的假设一致，即 PRP 给药对 AMSCs 向心肌细胞分化有影响。
