从大蒜中分离的凝集素（ASA）的生物学活性研究。
Investigations on the Biological Activity of Agglutinin (ASA) Isolated from Garlic.
机构信息
Department of Biotechnology, Panjab University, South Campus, Sector-25, Chandigarh-160014 India.
出版信息
Protein Pept Lett. 2022;29(6):555-566. doi: 10.2174/0929866529999220509122720.
BACKGROUND
Garlic (Allium sativum) from the family Amaryllidaceae is widely used in culinary and is reported to have potential anticancer, anti-diabetic, antimicrobial, and cardioprotective activities. Allium sativum agglutinin (ASA) is a bulb-type lectin (BTL) domaincontaining lectin isolated from garlic and has been studied for its various biological functions. Previous studies have reported the anti-cancer effects of ASA on histiocytic lymphoma (U937), promyelocytic leukemia (HL60), and oral cancer (KB).
METHODS
In this study, we have purified and characterized ASA and evaluated it for its anticancer effects on other cancer cell lines. MTT assay and FACS analysis was done to corroborate the anticancer findings against cervical (HeLa) and lung cancer (A549) cell lines.
RESULTS
IC value of 37 μg/ml in HeLa and a weak activity (26.4 ± 1.9% cellular inhibition at 100μg/ml treatment) in A549 were found in the MTT assay. FACS analysis further corroborated these findings and showed the apoptotic effects of ASA in these cell lines.
CONCLUSION
Anticancer activity for members of bulb-type lectin (BTL) domain-containing lectins has been widely reported, and we hope that our study forms a basis for the development of ASA as a therapeutic agent.
背景
百合科大蒜（Allium sativum）广泛用于烹饪，并据报道具有潜在的抗癌、抗糖尿病、抗菌和心脏保护作用。大蒜凝集素（ASA）是一种从大蒜中分离出的含 bulb-type lectin（BTL）结构域的凝集素，其各种生物学功能已得到研究。先前的研究报告了 ASA 对组织细胞淋巴瘤（U937）、早幼粒细胞白血病（HL60）和口腔癌（KB）的抗癌作用。
方法
在这项研究中，我们对 ASA 进行了纯化和表征，并评估了它对其他癌细胞系的抗癌作用。通过 MTT 检测和 FACS 分析证实了 ASA 对宫颈癌（HeLa）和肺癌（A549）细胞系的抗癌作用。
结果
在 MTT 检测中，HeLa 的 IC 值为 37μg/ml，A549 的活性较弱（100μg/ml 处理时细胞抑制率为 26.4±1.9%）。FACS 分析进一步证实了这些发现，并显示了 ASA 在这些细胞系中的凋亡作用。
结论
含 bulb-type lectin（BTL）结构域的凝集素成员的抗癌活性已得到广泛报道，我们希望我们的研究为 ASA 作为治疗剂的开发奠定基础。
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