利用杂交敏感双链荧光探针检测丝切蛋白信使核糖核酸
Detection of cofilin mRNA by hybridization-sensitive double-stranded fluorescent probes.
作者信息
Lee Ha Jung, Go Gui Han, Ro Jong Jin, Kim Byeang Hyean
机构信息
Department of Chemistry, Division of Advanced Materials Science, Pohang University of Science and Technology (POSTECH) Pohang 37673 Republic of Korea
出版信息
RSC Adv. 2018 Feb 16;8(14):7514-7517. doi: 10.1039/c7ra13349a. eCollection 2018 Feb 14.
Abstract
We have developed hybridization-sensitive fluorescent oligonucleotide probes that, in the presence of quencher strands, undergo efficient fluorescence quenching through the formation of partial DNA/DNA duplexes. In the presence of target RNA, rapid displacement of the quencher strands results in highly enhanced fluorescence.
摘要
我们开发了对杂交敏感的荧光寡核苷酸探针，在存在淬灭链的情况下，通过形成部分DNA/DNA双链体实现高效荧光淬灭。在存在靶RNA的情况下，淬灭链的快速置换导致荧光高度增强。
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