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利用杂交敏感双链荧光探针检测丝切蛋白信使核糖核酸

Detection of cofilin mRNA by hybridization-sensitive double-stranded fluorescent probes.

作者信息

Lee Ha Jung, Go Gui Han, Ro Jong Jin, Kim Byeang Hyean

机构信息

Department of Chemistry, Division of Advanced Materials Science, Pohang University of Science and Technology (POSTECH) Pohang 37673 Republic of Korea

出版信息

RSC Adv. 2018 Feb 16;8(14):7514-7517. doi: 10.1039/c7ra13349a. eCollection 2018 Feb 14.

DOI:10.1039/c7ra13349a
PMID:35539109
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9078427/
Abstract

We have developed hybridization-sensitive fluorescent oligonucleotide probes that, in the presence of quencher strands, undergo efficient fluorescence quenching through the formation of partial DNA/DNA duplexes. In the presence of target RNA, rapid displacement of the quencher strands results in highly enhanced fluorescence.

摘要

我们开发了对杂交敏感的荧光寡核苷酸探针，在存在淬灭链的情况下，通过形成部分DNA/DNA双链体实现高效荧光淬灭。在存在靶RNA的情况下，淬灭链的快速置换导致荧光高度增强。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb46/9078427/0cd3fbc49e7c/c7ra13349a-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb46/9078427/7edf493d04e2/c7ra13349a-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb46/9078427/812d008ed870/c7ra13349a-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb46/9078427/13e8168c157f/c7ra13349a-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb46/9078427/0cd3fbc49e7c/c7ra13349a-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb46/9078427/7edf493d04e2/c7ra13349a-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb46/9078427/812d008ed870/c7ra13349a-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb46/9078427/13e8168c157f/c7ra13349a-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb46/9078427/0cd3fbc49e7c/c7ra13349a-f4.jpg

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本文引用的文献

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Org Biomol Chem. 2017 Aug 30;15(34):7165-7172. doi: 10.1039/c7ob01703k.
2
Synthesis and hybridization properties of oligonucleotide analogues with novel acyclic triazole internucleotide linkages.具有新型无环三唑核苷酸间连接的寡核苷酸类似物的合成与杂交特性
Bioorg Chem. 2017 Jun;72:161-167. doi: 10.1016/j.bioorg.2017.04.004. Epub 2017 Apr 12.
3
Localization of cofilin mRNA to the leading edge of migrating cells promotes directed cell migration.
丝切蛋白信使核糖核酸定位于迁移细胞的前沿，促进细胞定向迁移。
J Cell Sci. 2015 May 15;128(10):1922-33. doi: 10.1242/jcs.163972. Epub 2015 Apr 23.
4
Roles of cofilin in development and its mechanisms of regulation.丝切蛋白在发育中的作用及其调控机制。
Dev Growth Differ. 2015 May;57(4):275-90. doi: 10.1111/dgd.12213. Epub 2015 Apr 10.
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Rationally designed molecular beacons for bioanalytical and biomedical applications.用于生物分析和生物医学应用的合理设计的分子信标。
Chem Soc Rev. 2015 May 21;44(10):3036-55. doi: 10.1039/c5cs00020c. Epub 2015 Mar 17.
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