采用新的浓缩方法检测尿样中的诱变活性。

Detection of mutagenic activity in urine samples using a new concentration procedure.

作者信息

Duverger-van Bogaert M, Stecca C, Léonard A

出版信息

Mutat Res. 1987 May;178(1):21-4. doi: 10.1016/0027-5107(87)90081-9.

Abstract

A study performed with cyclophosphamide (CP) and nor-nitrogen mustard (NNM), one of its main urinary metabolites, has shown that separation on Polygosil C-18 resin is preferable to one on XAD-2 resin as a means of concentrating the mutagenic activity present in urine of rats given cytostatics such as CP. Mutagenic activity was detected, using Salmonella typhimurium tester strain TA1535. While NNM is irreversibly bound to XAD-2 resin, it can be recovered after elution with methanol on Polygosil C-18. The better efficacity of Polygosil C-18 in concentrating CP and its metabolite(s) was confirmed with experiments with urine of rats treated with increasing doses of CP.

摘要

一项使用环磷酰胺（CP）及其主要尿液代谢物之一的去甲氮芥（NNM）进行的研究表明，对于浓缩给予细胞抑制剂（如CP）的大鼠尿液中存在的诱变活性而言，在聚硅氧烷C - 18树脂上进行分离比在XAD - 2树脂上进行分离更可取。使用鼠伤寒沙门氏菌测试菌株TA1535检测诱变活性。虽然NNM与XAD - 2树脂不可逆结合，但在用甲醇洗脱后，它可以在聚硅氧烷C - 18上回收。用给予递增剂量CP的大鼠尿液进行的实验证实了聚硅氧烷C - 18在浓缩CP及其代谢物方面具有更好的效果。