大鼠皮肤接触1,3-二氨基苯后尿液中的致突变代谢物。

Mutagenic metabolites in rat urine after dermal exposure to 1,3-diaminobenzene.

作者信息

Clemmensen S, Lam H, Büchert A

出版信息

Arch Toxicol Suppl. 1985;8:403-6. doi: 10.1007/978-3-642-69928-3_87.

Abstract

Amberlite XAD-2 purified urine extracts from rats treated dermally with 1,3-diaminobenzene were fractionated by HPLC and the metabolites were identified by GC-MS. Mutagenic activity in Salmonella typhimurium TA 98 was equally distributed between the two fractions, none of which contained the parent amine. The first fraction, eluted between 2-7 minutes, contained only a very small amount of presently unidentified metabolites, while the second fraction eluted between 14-19 minutes contained N,N'-diacetyl-1,3-diaminobenzene. Both fractions showed minor, but still significant activity even without metabolic activation. A non-mutagenic fraction, eluted between 10-12 minutes contained N-acetyl -acetyl-1,3-diaminobenzene and N,N'-diacetyl-2,4-diaminophenol.

摘要

用1,3 - 二氨基苯经皮肤处理大鼠后，其尿液提取物经Amberlite XAD - 2纯化，通过高效液相色谱（HPLC）进行分离，并通过气相色谱 - 质谱联用仪（GC - MS）鉴定代谢产物。鼠伤寒沙门氏菌TA 98中的诱变活性在两个馏分中均匀分布，两个馏分均不含母体胺。第一个馏分在2 - 7分钟之间洗脱，仅含有极少量目前尚未鉴定的代谢产物，而第二个馏分在14 - 19分钟之间洗脱，含有N,N'-二乙酰基-1,3 - 二氨基苯。即使没有代谢活化，两个馏分均显示出轻微但仍显著的活性。在10 - 12分钟之间洗脱的一个非诱变馏分含有N - 乙酰 - 乙酰基-1,3 - 二氨基苯和N,N'-二乙酰基-2,4 - 二氨基苯酚。