Liu Xiaolin, Ni Shou-Qing, Guo Wenshan, Wang Zhibin, Ahmad Hafiz Adeel, Gao Baoyu, Fang Xu
Shandong Provincial Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University No. 27 Shanda South Road Jinan 250100 Shandong PR China
Centre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney Sydney NSW 2007 Australia.
RSC Adv. 2018 Jul 5;8(43):24305-24311. doi: 10.1039/c8ra03032d. eCollection 2018 Jul 2.
In this study, greenhouse gas emissions and microbial community succession during the realization of the partial nitrification (PN) process were studied. The results show that NO emission mainly occurred in the aerobic stage and the PN reactor released about 20 mg of NO gas each cycle. There is a positive correlation between the dissolved NO concentration and the temperature of a typical cycle. High-throughput sequencing was used to illustrate succession in the microbial community structure. The most significant microfloral change during the PN startup process was that some aerobic bacteria were relatively enriched and some anaerobic bacteria were weeded out. The ammonia oxidizing bacteria (AOB) like Nitrosomonadaceae were enriched on account of the suitable external environment. whose main function is denitrification declined and (anammox) showed the same tendency. This study comprehensively demonstrates the fluctuations of dissolved and emitted NO while researching the succession of the microbial community in the culture of the PN process.
在本研究中，对实现部分硝化（PN）过程期间的温室气体排放和微生物群落演替进行了研究。结果表明，NO排放主要发生在好氧阶段，PN反应器每个循环释放约20mg的NO气体。典型循环中溶解态NO浓度与温度之间存在正相关关系。采用高通量测序来说明微生物群落结构的演替。PN启动过程中最显著的微生物区系变化是一些好氧细菌相对富集，一些厌氧细菌被淘汰。由于适宜的外部环境，诸如亚硝化单胞菌科等氨氧化细菌（AOB）得以富集。其主要功能为反硝化作用的细菌数量下降，厌氧氨氧化菌（anammox）也呈现相同趋势。本研究在研究PN过程培养中微生物群落演替的同时，全面展示了溶解态和排放态NO的波动情况。
