DNA-induced synthesis of biomimetic enzyme for sensitive detection of superoxide anions released from live cell.

In this work, we successfully fabricate a rapid, sensitive sensor for the detection of superoxide anions O˙ based on graphene/DNA/Mn(PO) biomimetic enzyme. In the design, graphene is served as excellent carrier to improve the catalysis of Mn(PO) nanoparticles; and DNA adsorbed on graphene acts as medium to assist the growth of Mn(PO) on graphene. The fabricated graphene/DNA/Mn(PO) composites exhibit excellently electrochemical activity, significantly decrease the response time and increase the sensitivity of the sensor towards O˙. The successful detection of O˙ released from cancer cell demonstrated its potential applications in biology and medicine.