DNA诱导合成用于灵敏检测活细胞释放的超氧阴离子的仿生酶。
DNA-induced synthesis of biomimetic enzyme for sensitive detection of superoxide anions released from live cell.
作者信息
Ding Ailing, Wang Bin, Ma Xiaoqing, Diao Jianglin, Zheng Jiushang, Chen Jiucun, Li Changming
机构信息
Institute for Clean Energy & Advanced Materials, Faculty of Materials and Energy, Southwest University Chongqing 400715 P. R. China
出版信息
RSC Adv. 2018 Apr 3;8(22):12354-12359. doi: 10.1039/c7ra12962a. eCollection 2018 Mar 26.
In this work, we successfully fabricate a rapid, sensitive sensor for the detection of superoxide anions O˙ based on graphene/DNA/Mn(PO) biomimetic enzyme. In the design, graphene is served as excellent carrier to improve the catalysis of Mn(PO) nanoparticles; and DNA adsorbed on graphene acts as medium to assist the growth of Mn(PO) on graphene. The fabricated graphene/DNA/Mn(PO) composites exhibit excellently electrochemical activity, significantly decrease the response time and increase the sensitivity of the sensor towards O˙. The successful detection of O˙ released from cancer cell demonstrated its potential applications in biology and medicine.
在本工作中，我们成功制备了一种基于石墨烯/DNA/Mn(PO)仿生酶的用于检测超氧阴离子O˙的快速、灵敏传感器。在设计中，石墨烯作为优良载体以提高Mn(PO)纳米颗粒的催化作用；吸附在石墨烯上的DNA作为介质辅助Mn(PO)在石墨烯上生长。所制备的石墨烯/DNA/Mn(PO)复合材料表现出优异的电化学活性，显著缩短了响应时间并提高了传感器对O˙的灵敏度。成功检测到癌细胞释放的O˙证明了其在生物学和医学中的潜在应用。
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