• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过开环反应制备的用于基因递送和细胞成像的光致发光氟掺杂碳点。

Photoluminescent F-doped carbon dots prepared by ring-opening reaction for gene delivery and cell imaging.

作者信息

Luo Tian-Ying, He Xi, Zhang Ji, Chen Ping, Liu Yan-Hong, Wang Hai-Jiao, Yu Xiao-Qi

机构信息

Key Laboratory of Green Chemistry and Technology, Ministry of Education, College of Chemistry, Sichuan University Chengdu 610064 P. R. China

出版信息

RSC Adv. 2018 Feb 7;8(11):6053-6062. doi: 10.1039/c7ra13607b. eCollection 2018 Feb 2.

DOI:10.1039/c7ra13607b
PMID:35539571
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9078219/
Abstract

Carbon dots (CDs) are photoluminescent nanoparticles with distinctive properties, having great potential in nano-biomaterial systems such as gene/drug delivery vectors and cell imaging agents. Fluorine-doped CD C-6F was prepared by a one-step ring-opening polymerization-dehydrative carbonization (RPDC) approach based on low molecular weight polyethyleneimine (PEI, 600 Da) and fluorinated diglycidyl ethers, while the non-fluorinated counterpart C-6H and the CD prepared from PEI 600 Da solely (C-600) were also prepared for comparison. TEM, FT-IR and XPS were performed to determine the compositions and surface states of the CDs. cell experiment results reveal that the CDs prepared from RPDC approach have much higher transfection efficiency and cellular uptake than PEI 600 contrasts in various cell lines. Compared to non-fluorinated C-6H, C-6F exhibited distinctly higher transfection efficiency, and up to 30 and 260 times higher efficiency than PEI 25 kDa could be achieved in the absence and presence of serum, respectively, indicating the advantage of F-doping. Besides, these CDs exhibit good cell imaging capability under single wavelength excitation, making the materials suitable for cellular tracking and transfection mechanism studies. These results demonstrate that fluorine-doping is an efficient approach to obtained CD gene vectors with high efficiency and serum tolerance.

摘要

碳点（CDs）是具有独特性质的光致发光纳米颗粒，在纳米生物材料系统中具有巨大潜力，如基因/药物递送载体和细胞成像剂。基于低分子量聚乙烯亚胺（PEI，600 Da）和氟化二缩水甘油醚，通过一步开环聚合-脱水碳化（RPDC）方法制备了氟掺杂的CD C-6F，同时还制备了非氟化的对应物C-6H以及仅由PEI 600 Da制备的CD（C-600）用于比较。进行了透射电子显微镜（TEM）、傅里叶变换红外光谱（FT-IR）和X射线光电子能谱（XPS）分析以确定碳点的组成和表面状态。细胞实验结果表明，通过RPDC方法制备的碳点在各种细胞系中的转染效率和细胞摄取量比PEI 600对照物高得多。与非氟化的C-6H相比，C-6F表现出明显更高的转染效率，在无血清和有血清的情况下，其效率分别比PEI 25 kDa高30倍和260倍，表明了氟掺杂的优势。此外，这些碳点在单波长激发下表现出良好的细胞成像能力，使该材料适用于细胞追踪和转染机制研究。这些结果表明，氟掺杂是获得具有高效性和血清耐受性的CD基因载体的有效方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45d5/9078219/f384e8748a5a/c7ra13607b-f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45d5/9078219/6c9f660e19a7/c7ra13607b-s1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45d5/9078219/5f1a684dfe76/c7ra13607b-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45d5/9078219/8bb0dc032721/c7ra13607b-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45d5/9078219/c752b78f7595/c7ra13607b-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45d5/9078219/380c3d643e7a/c7ra13607b-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45d5/9078219/053cd64aa5a0/c7ra13607b-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45d5/9078219/dcf2d9db24eb/c7ra13607b-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45d5/9078219/ae739fea5fc9/c7ra13607b-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45d5/9078219/2401a1475c31/c7ra13607b-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45d5/9078219/f384e8748a5a/c7ra13607b-f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45d5/9078219/6c9f660e19a7/c7ra13607b-s1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45d5/9078219/5f1a684dfe76/c7ra13607b-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45d5/9078219/8bb0dc032721/c7ra13607b-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45d5/9078219/c752b78f7595/c7ra13607b-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45d5/9078219/380c3d643e7a/c7ra13607b-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45d5/9078219/053cd64aa5a0/c7ra13607b-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45d5/9078219/dcf2d9db24eb/c7ra13607b-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45d5/9078219/ae739fea5fc9/c7ra13607b-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45d5/9078219/2401a1475c31/c7ra13607b-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45d5/9078219/f384e8748a5a/c7ra13607b-f9.jpg

相似文献

1
Photoluminescent F-doped carbon dots prepared by ring-opening reaction for gene delivery and cell imaging.通过开环反应制备的用于基因递送和细胞成像的光致发光氟掺杂碳点。
RSC Adv. 2018 Feb 7;8(11):6053-6062. doi: 10.1039/c7ra13607b. eCollection 2018 Feb 2.
2
Hydrophobically modified carbon dots as a multifunctional platform for serum-resistant gene delivery and cell imaging.疏水改性碳点作为一种多功能平台用于抗血清基因传递和细胞成像。
Biomater Sci. 2020 Jul 7;8(13):3730-3740. doi: 10.1039/d0bm00651c. Epub 2020 Jun 5.
3
One-step fabrication of functional carbon dots with long wavelength emission for gene delivery and bio-imaging.一步法制备具有长波长发射的功能化碳点用于基因传递和生物成像。
J Mater Chem B. 2021 Oct 20;9(40):8518-8529. doi: 10.1039/d1tb01622a.
4
Linear polycations by ring-opening polymerization as non-viral gene delivery vectors.通过开环聚合制备的线性聚阳离子作为非病毒基因传递载体。
Biomaterials. 2013 Jul;34(21):5391-401. doi: 10.1016/j.biomaterials.2013.03.083. Epub 2013 Apr 10.
5
Diol glycidyl ether-bridged low molecular weight PEI as potential gene delivery vehicles.二醇缩水甘油醚桥连的低分子量聚乙烯亚胺作为潜在的基因传递载体。
J Mater Chem B. 2015 Apr 7;3(13):2660-2670. doi: 10.1039/c4tb02106a. Epub 2015 Feb 26.
6
Gadolinium-doped carbon dots as nano-theranostic agents for MR/FL diagnosis and gene delivery.掺钆碳点作为磁共振/荧光诊断和基因传递的纳米治疗一体化试剂。
Nanoscale. 2019 Jul 21;11(27):12973-12982. doi: 10.1039/c9nr03988k. Epub 2019 Jul 2.
7
Cross-linked polymers with fluorinated bridges for efficient gene delivery.具有氟化桥连结构的交联聚合物用于高效基因递送
J Mater Chem B. 2017 Nov 21;5(43):8542-8553. doi: 10.1039/c7tb02158e. Epub 2017 Oct 24.
8
One-step synthesis of photoluminescent carbon dots with excitation-independent emission for selective bioimaging and gene delivery.一步法合成具有激发独立发射的光致发光碳点，用于选择性生物成像和基因传递。
J Colloid Interface Sci. 2017 Apr 15;492:1-7. doi: 10.1016/j.jcis.2016.12.057. Epub 2016 Dec 24.
9
Photoluminescent and biodegradable polycitrate-polyethylene glycol-polyethyleneimine polymers as highly biocompatible and efficient vectors for bioimaging-guided siRNA and miRNA delivery.具有光致发光和可生物降解性的聚柠檬酸盐-聚乙二醇-聚乙烯亚胺聚合物作为高度生物相容性和高效的载体，用于生物成像引导的 siRNA 和 miRNA 递送。
Acta Biomater. 2017 May;54:69-80. doi: 10.1016/j.actbio.2017.02.034. Epub 2017 Feb 20.
10
AS1411 aptamer modified carbon dots via polyethylenimine-assisted strategy for efficient targeted cancer cell imaging.AS1411 适体通过聚乙烯亚胺辅助策略修饰碳点用于高效靶向癌细胞成像。
Cell Prolif. 2020 Jan;53(1):e12713. doi: 10.1111/cpr.12713. Epub 2019 Nov 5.

引用本文的文献

1
Heteroatom-doped carbon dots from medicinal plants as novel biomaterials for as-use biomedical applications in comparison with synthetic drug, zaltoprofen.药用植物源杂原子掺杂碳点作为新型生物材料在即时应用的生物医学中的应用与合成药物佐美酸的比较。
Sci Rep. 2024 Jun 7;14(1):13160. doi: 10.1038/s41598-024-63700-w.
2
Recent progress of emitting long-wavelength carbon dots and their merits for visualization tracking, target delivery and theranostics.发射长波长碳点的最新进展及其在可视化追踪、靶向递药和治疗中的优势。
Theranostics. 2023 May 21;13(9):3064-3102. doi: 10.7150/thno.80579. eCollection 2023.
3
Recent Developments in Heteroatom/Metal-Doped Carbon Dot-Based Image-Guided Photodynamic Therapy for Cancer.

本文引用的文献

1
Cross-linked polymers with fluorinated bridges for efficient gene delivery.具有氟化桥连结构的交联聚合物用于高效基因递送
J Mater Chem B. 2017 Nov 21;5(43):8542-8553. doi: 10.1039/c7tb02158e. Epub 2017 Oct 24.
2
Fluorination on polyethylenimine allows efficient 2D and 3D cell culture gene delivery.聚乙烯亚胺上的氟化可实现高效的二维和三维细胞培养基因递送。
J Mater Chem B. 2015 Jan 28;3(4):642-650. doi: 10.1039/c4tb01447b. Epub 2014 Nov 28.
3
Amphiphilic carbon dots as versatile vectors for nucleic acid and drug delivery.两亲性碳点作为核酸和药物传递的多功能载体。
基于杂原子/金属掺杂碳点的癌症图像引导光动力疗法的最新进展
Pharmaceutics. 2022 Sep 5;14(9):1869. doi: 10.3390/pharmaceutics14091869.
4
Halogen-Doped Carbon Dots: Synthesis, Application, and Prospects.卤化碳点：合成、应用及前景。
Molecules. 2022 Jul 20;27(14):4620. doi: 10.3390/molecules27144620.
5
Fluorescent Carbon Dot-Supported Imaging-Based Biomedicine: A Comprehensive Review.基于荧光碳点成像的生物医学：综述
Bioinorg Chem Appl. 2022 Apr 10;2022:9303703. doi: 10.1155/2022/9303703. eCollection 2022.
6
Gold Nanoparticles for Vectorization of Nucleic Acids for Cancer Therapeutics.金纳米粒子用于癌症治疗中核酸的载体化。
Molecules. 2020 Jul 31;25(15):3489. doi: 10.3390/molecules25153489.
7
[12]aneN-based multifunctional compounds as fluorescent probes and nucleic acids delivering agents.基于吖啶的多功能化合物作为荧光探针和核酸递药载体。
Drug Deliv. 2020 Dec;27(1):66-80. doi: 10.1080/10717544.2019.1704943.
8
Small molecules derived carbon dots: synthesis and applications in sensing, catalysis, imaging, and biomedicine.小分子衍生碳点：合成及在传感、催化、成像和生物医学中的应用。
J Nanobiotechnology. 2019 Aug 26;17(1):92. doi: 10.1186/s12951-019-0525-8.
Nanoscale. 2017 May 11;9(18):5935-5947. doi: 10.1039/c7nr01029j.
4
Efficient Gene Transfection through Inhibition of β-Sheet (Amyloid Fiber) Formation of a Short Amphiphilic Peptide by Gold Nanoparticles.通过金纳米粒子抑制短两亲肽的β-折叠（淀粉样纤维）形成实现高效基因转染。
Angew Chem Int Ed Engl. 2017 Jul 3;56(28):8083-8088. doi: 10.1002/anie.201700713. Epub 2017 Apr 10.
5
Bright Multicolor Bandgap Fluorescent Carbon Quantum Dots for Electroluminescent Light-Emitting Diodes.用于电致发光发光二极管的亮多彩带隙荧光碳量子点。
Adv Mater. 2017 Jan;29(3). doi: 10.1002/adma.201604436. Epub 2016 Nov 23.
6
Near-infrared emissive carbon dots for two-photon fluorescence bioimaging.用于双光子荧光生物成像的近红外发射碳点。
Nanoscale. 2016 Oct 6;8(39):17350-17356. doi: 10.1039/c6nr05878g.
7
Treating Immunodeficiency through HSC Gene Therapy.通过造血干细胞基因治疗治疗免疫缺陷。
Trends Mol Med. 2016 Apr;22(4):317-327. doi: 10.1016/j.molmed.2016.02.002. Epub 2016 Mar 15.
8
Full-Color Light-Emitting Carbon Dots with a Surface-State-Controlled Luminescence Mechanism.具有表面态控制发光机制的全彩色发光碳点。
ACS Nano. 2016 Jan 26;10(1):484-91. doi: 10.1021/acsnano.5b05406. Epub 2015 Dec 8.
9
High DNA-Binding Affinity and Gene-Transfection Efficacy of Bioreducible Cationic Nanomicelles with a Fluorinated Core.含氟核的生物还原型阳离子纳米胶束具有高 DNA 结合亲和力和基因转染效率。
Angew Chem Int Ed Engl. 2016 Jan 11;55(2):755-9. doi: 10.1002/anie.201508695. Epub 2015 Nov 20.
10
Nucleus-staining with biomolecule-mimicking nitrogen-doped carbon dots prepared by a fast neutralization heat strategy.通过快速中和热策略制备的生物分子模拟氮掺杂碳点对细胞核进行染色。
Chem Commun (Camb). 2015 Dec 11;51(95):16956-9. doi: 10.1039/c5cc06304c. Epub 2015 Oct 7.