He Yingcong, Zhu Ting, Liu Lei, Shi Xuetao, Lin Zhengmei
Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology Guangzhou 510055 China
National Engineering Research Centre for Tissue Restoration and Reconstruction, School of Material Science and Engineering, South China University of Technology Guangzhou 510640 PR China
RSC Adv. 2018 May 8;8(30):16762-16772. doi: 10.1039/c8ra01872c. eCollection 2018 May 3.
Phosphorylated materials are attractive candidates for bone regeneration because they may facilitate the construction of a phosphorylated bone extracellular matrix (ECM) to build a beneficial environment for bone formation. Here, we designed and synthesized a new phosphorylated material, collagen type I phosphorylated with alendronate sodium (Col-Aln), based on the biodegradable osteoconductive collagen backbone. Col-Aln can distinctly accelerate mineralization in simulated body fluid. Col-Aln showed good biocompatibility with bone marrow mesenchymal stem cells (BMSCs) and promoted their adhesion as well as the osteogenic differentiation of BMSCs more effectively than did pure collagen. Furthermore, collagen and Col-Aln scaffolds implanted into a critical-sized rat cranial defect for 4 and 8 weeks were shown to degrade and helped to facilitate bone growth in the defect, while the phosphate-containing Col-Aln scaffold significantly promoted new bone formation. Col-Aln provides a new strategy to integrate bioactive phosphate molecules covalent grafting onto biopolymers and has promise for bone regeneration applications.
磷酸化材料是骨再生的理想候选材料，因为它们可能有助于构建磷酸化的骨细胞外基质（ECM），为骨形成营造有利环境。在此，我们基于可生物降解的骨传导性胶原骨架，设计并合成了一种新的磷酸化材料，即阿仑膦酸钠磷酸化的I型胶原（Col-Aln）。Col-Aln能显著加速模拟体液中的矿化作用。Col-Aln与骨髓间充质干细胞（BMSC）表现出良好的生物相容性，并且比纯胶原更有效地促进了BMSC的黏附及其成骨分化。此外，植入大鼠临界尺寸颅骨缺损处4周和8周的胶原和Col-Aln支架显示出降解，并有助于促进缺损处的骨生长，而含磷酸盐的Col-Aln支架显著促进了新骨形成。Col-Aln提供了一种将生物活性磷酸分子共价接枝到生物聚合物上的新策略，在骨再生应用方面具有前景。
