State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China.
State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China.
Mater Sci Eng C Mater Biol Appl. 2017 Feb 1;71:118-124. doi: 10.1016/j.msec.2016.09.085. Epub 2016 Sep 30.
In this study, novel poly(γ-benzyl l-glutamate)/poly(lactic-co-glycolic acid)/bioglass (PBLG/PLGA/BG) composite scaffolds with different weight ratios were fabricated using a negative NaCl-templating method. The morphology, compression modulus and degradation kinetics of the scaffolds were characterized. The results showed that the PBLG/PLGA/BG composite scaffolds with a weight ratio of 5:5:1, namely PBLG5PLGA5BG composite scaffolds, displayed a pore size range of 50-500μm, high compressive modulus (566.6±8.8kPa), suitable glass transition temperature (46.8±0.2°C) and low degradation rate (>8weeks). The in vitro biocompatibility of the scaffolds was evaluated with MC3T3-E1 cells by live-dead staining, MTT and ALP activity assays. The obtained results indicated that the PBLG5PLGA5BG composite scaffolds were more conducive to the adhesion, proliferation and osteoblastic differentiation of MC3T3-E1 cells than PBLG and PBLG/PLGA composite scaffolds. The in vivo biocompatibility of the scaffolds was evaluated in both SD rat subcutaneous model and rabbit tibia defect model. The results of H&E, Masson's trichrome and CD34 staining assays demonstrated that the PBLG5PLGA5BG composite scaffolds allowed the ingrowth of tissue and microvessels more effectively than PBLG/PLGA composite scaffolds. The results of digital radiography confirmed that the PBLG5PLGA5BG composite scaffolds significantly improved in vivo osteogenesis. Collectively, the PBLG5PLGA5BG composite scaffolds could be a promising candidate for tissue engineering applications.
在这项研究中，我们采用负相 NaCl 模板法制备了不同重量比的新型聚（γ-苄基 L-谷氨酸酯）/聚（乳酸-共-乙醇酸）/生物玻璃（PBLG/PLGA/BG）复合支架。对支架的形态、压缩模量和降解动力学进行了表征。结果表明，重量比为 5:5:1 的 PBLG/PLGA/BG 复合支架（即 PBLG5PLGA5BG 复合支架）具有 50-500μm 的孔径范围、较高的压缩模量（566.6±8.8kPa）、合适的玻璃化转变温度（46.8±0.2°C）和较低的降解速率（>8 周）。通过活死染色、MTT 和 ALP 活性测定评估了支架的体外生物相容性。结果表明，与 PBLG 和 PBLG/PLGA 复合支架相比，PBLG5PLGA5BG 复合支架更有利于 MC3T3-E1 细胞的黏附、增殖和成骨分化。通过 SD 大鼠皮下模型和兔胫骨缺损模型评估了支架的体内生物相容性。H&E、Masson 三色和 CD34 染色结果表明，与 PBLG/PLGA 复合支架相比，PBLG5PLGA5BG 复合支架更有利于组织和微血管的长入。数字射线摄影结果证实，PBLG5PLGA5BG 复合支架可显著促进体内成骨。综上所述，PBLG5PLGA5BG 复合支架有望成为组织工程应用的候选材料。
