Zheng Zhi, Liu Pingyi, Xu Liting, Peng Zhiqiang, Zhang Yayue, Chen Xinyi, Hou Li, Cui Wenhao, Tou Fangfang, Rao Jun, Fan Xing
Jiangxi Cancer Hospital, Jiangxi Cancer Center, Medical College of Nanchang University Nanchang 330029 P. R. China
Laboratory of Department of Hematology, Yishui Central Hospital of Linyi Linyi Shandong Province 276400 P. R.China.
RSC Adv. 2018 Apr 18;8(26):14445-14453. doi: 10.1039/c7ra13298k. eCollection 2018 Apr 17.
Acute myeloid leukemia (AML) is a group of hematological malignancies causing high mortality around the world. However, the treatment of AML is still one of the most formidable challenges. In this study, we employed a well-established global metabolic profiling platform, which combined ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) with gas chromatography mass spectrometry (GC-MS) to investigate the metabolic alterations associated with salvage chemotherapy on 10 refractory acute myeloid leukemia (RAML) patients. A total of 390 metabolites were identified from 20 serum samples obtained from all 10 patients before and post salvage chemotherapy. The metabolomics profile was found to be very heterogeneous across the RAML patients. The results showed very subtle metabolic differences upon one-time chemotherapy treatment for an individual patient. Only 9 metabolites including imidazole lactate, glycerol 3-phosphate, three fatty acids, and four lysolipids in the blood serum were significantly changed before and post chemotherapy, suggesting their important roles during the development of RAML. This study may not only provide new insight into the metabolomics features in RAML patients, but also have relevance to improve the treatment and outcome of RAML.
急性髓系白血病（AML）是一类血液系统恶性肿瘤，在全球范围内导致高死亡率。然而，AML的治疗仍然是最严峻的挑战之一。在本研究中，我们采用了一个成熟的全球代谢谱分析平台，该平台将超高效液相色谱-串联质谱（UPLC-MS/MS）与气相色谱-质谱（GC-MS）相结合，以研究10例难治性急性髓系白血病（RAML）患者挽救化疗相关的代谢改变。从所有10例患者挽救化疗前后采集的20份血清样本中总共鉴定出390种代谢物。发现RAML患者的代谢组学特征非常异质。结果显示，单次化疗治疗后个体患者的代谢差异非常细微。血清中只有9种代谢物，包括咪唑乳酸、3-磷酸甘油、三种脂肪酸和四种溶血脂质，在化疗前后有显著变化，表明它们在RAML发生过程中起重要作用。本研究不仅可能为RAML患者的代谢组学特征提供新的见解，而且可能与改善RAML的治疗和预后相关。
