Li Xiaofeng, Xu Haixia, Zhao Guanglei, Wu Hui, Yu Yigang, Lai Furao, Xiao Xinglong
State Key Laboratory of Pulp and Paper Engineering, South China University of Technology Wushan Road 381 Guangzhou 510640 China
School of Food Science and Engineering, South China University of Technology Guangzhou 510640 China
RSC Adv. 2018 Mar 13;8(18):10081-10088. doi: 10.1039/c8ra00595h. eCollection 2018 Mar 5.
Acylation modification of phenol glycosides is currently of great interest due to the improved bioavailability and multiple functions. In this work, mono- or diesters of arbutin, an important phenol glycoside derivative, can be controllably synthesized by using whole-cell biocatalytic systems. Among fourteen microbial strains selected, cells showed the best catalytic activity and high organic solvent tolerance. Compared with the best pure solvent tetrahydrofuran, the use of a binary solvent pyridine-isooctane gave a slightly lower conversion (98.3% 97.2%) and selectivity (85.3% 80.5%) and much higher substrate solubility (37.1 214.0 mg mL), in a 24 h bioconversion of arbutin with a VP-arbutin molar ratio of 15 and whole cell dosage of 30 mg mL. The production of various arbutin esters with different fatty acid chain lengths can be realized by using this whole-cell strategy, with the substrate conversion and 6'-regioselectivity of 54.1-98.3% and 83.2-99.0%, respectively.
由于生物利用度的提高和多种功能，酚苷的酰化修饰目前备受关注。在这项工作中，重要的酚苷衍生物熊果苷的单酯或二酯可以通过全细胞生物催化系统可控合成。在所筛选的14株微生物菌株中，[具体菌株名称]细胞表现出最佳的催化活性和高有机溶剂耐受性。在熊果苷的24小时生物转化中，当VP-熊果苷摩尔比为15且全细胞用量为30mg/mL时，与最佳纯溶剂四氢呋喃相比，使用二元溶剂吡啶-异辛烷时转化率（98.3%对97.2%）和选择性（85.3%对80.5%）略低，但底物溶解度（37.1对214.0mg/mL）高得多。通过这种全细胞策略可以实现不同脂肪酸链长度的各种熊果苷酯的生产，底物转化率和6'-区域选择性分别为54.1-98.3%和83.2-99.0%。
