Żbikowska Beata, Franiczek Roman, Sowa Alina, Połukord Grażyna, Krzyżanowska Barbara, Sroka Zbigniew
1 Department of Pharmacognosy, Faculty of Pharmacy, Wrocław Medical University , Wrocław, Poland .
2 Department of Microbiology, Faculty of Medicine, Wrocław Medical University , Wrocław, Poland .
Microb Drug Resist. 2017 Sep;23(6):771-780. doi: 10.1089/mdr.2016.0251. Epub 2017 Jan 24.
An important focus of modern medicine is the search for new substances and strategies to combat infectious diseases, which present an increasing threat due to the growth of bacterial resistance to antibiotics. Another problem concerns free radicals, which in excess can cause several serious diseases. An alternative to chemical synthesis of antimicrobial and antiradical compounds is to find active substances in plant raw materials. We prepared extracts from leaves of five species of the genus Bergenia: B. purpurascens, B. cordifolia, B. ligulata, B. crassifolia, and B. ciliata. Antimicrobial and antiradical features of extracts and raw materials were assessed, and the quantities of phenolic compounds were determined. We also evaluated, using high-performance liquid chromatography, the amounts of arbutin and hydroquinone, compounds related to antimicrobial activity of these raw materials. The strongest antiradical properties were shown by leaves of B. crassifolia and B. cordifolia, the lowest by leaves of B. ciliata. The antiradical activity of extracts showed a strong positive correlation with the amount of phenols. All raw materials have significant antimicrobial properties. Among them, the ethyl acetate extracts were the most active. Antimicrobial activity very weakly correlated with the amount of arbutin, but correlated very strongly with the contents of both hydroquinone and phenolic compounds. Additional experiments using artificially prepared mixtures of phenolic compounds and hydroquinone allowed us to conclude that the most active antimicrobial substance is hydroquinone.
现代医学的一个重要关注点是寻找对抗传染病的新物质和新策略，由于细菌对抗生素的耐药性不断增强，传染病构成的威胁日益增大。另一个问题涉及自由基，自由基过量会引发多种严重疾病。抗菌和抗自由基化合物化学合成的替代方法是在植物原料中寻找活性物质。我们从岩白菜属的五个物种的叶子中制备了提取物：紫花岩白菜、心叶岩白菜、舌叶岩白菜、厚叶岩白菜和睫毛岩白菜。评估了提取物和原料的抗菌和抗自由基特性，并测定了酚类化合物的含量。我们还使用高效液相色谱法评估了熊果苷和对苯二酚的含量，这两种化合物与这些原料的抗菌活性有关。厚叶岩白菜和心叶岩白菜的叶子表现出最强的抗自由基特性，睫毛岩白菜的叶子表现出的抗自由基特性最弱。提取物的抗自由基活性与酚类物质的含量呈强正相关。所有原料都具有显著的抗菌特性。其中，乙酸乙酯提取物活性最强。抗菌活性与熊果苷的含量相关性非常弱，但与对苯二酚和酚类化合物的含量相关性非常强。使用人工制备的酚类化合物和对苯二酚混合物进行的额外实验使我们得出结论，最具活性的抗菌物质是对苯二酚。
